摘要
用兔抗牛轮状病毒(NCDV株)IgG致敏绵羊红细胞进行反向间接血凝(RIHA)试验,直接检测105份犊牛腹泻粪样中的轮状病毒,查出阳性39份,阳性检出率为37.1％,与电镜检查,核酸PAGE和ELISA相比较,符合率分别为83.3％,91.7％和98.1％。同时还建立了用反向间接血凝抑制(RIHI)试验检测人和动物血清轮状病毒抗体的方法。两法特异性好,简便快速,易于推广。
A reverse indirect hemagglutination ( RIHA ) test was used for detecting rotavirus antigen directly from 105 fecal samples of diarrheal calves. Of these 39 ( 37.1% ) were positive. The correspondent rate of RIHA with EM, PAGE of genome RNA and ELISA was 83.3%, 91.7% and 98.1% respectively. Reverse indirect hemagglutination inhibition ( RIHI ) test was used in the detection of rotavirus serum antibody in cattle. These tests are rapid, simple, sensitive, and specific. It is more convenient for use in the clinics and for epidemiological survey.
出处
《病毒学报》
CAS
CSCD
北大核心
1989年第1期68-71,共4页
Chinese Journal of Virology
