摘要
目的 :证明血灵对血管平滑肌钙通道阻滞作用。方法 :用累积浓度法依次加入去甲肾上腺素(NE) ,得NE引起的血管平滑肌收缩的量效曲线 ,收缩张力恢复正常后加入血灵 ,测定给药后量效曲线 ,观察血灵对NE引起的血管平滑肌收缩的影响 ;同法观察血灵对KCl、CaCl2 引起的血管平滑肌收缩的影响 ,及对NE引起的依赖于细胞内钙与细胞外钙收缩反应的影响。结果 :血灵能够使NE和CaCl2收缩血管平滑肌的量效曲线下移 ,作用性质与维拉帕米(Ver)相似 ,呈非竞争性拮抗反应 ;血灵对NE引起的依赖于细胞内钙收缩有明显的抑制作用 ,而对外钙收缩则无明显的抑制。结论 :血灵的作用类似于钙通道阻滞剂维拉帕米 。
Objective: To confirm the effect of Xueling on blocking the inward flow of calcium Methods: The dose effect curve of vascular smooth muscle's contraction was obtained by adding an accumulative series of NE's concentration Then Xueling was added after the strain of vascular smooth muscle recuperated, measured the NE's dose effect curve to observe the contraction of the vascular smooth muscle; in the same way were Xueling's effect on the vascular smooth muscle observed by adding a certain concentration of KCl and CaCl2, and the effect on the contraction of the vascular smooth muscle depending on the intracellular calcium and extracellular calcium as observed by adding NE Results: Xueling would enable to shift down the dose effect curve of vascular smooth muscle induced by NE and CaCl2 and its working mechanism was similar to that of Ver in the way of no competitive calcium blocking Xueling had a repressive effect on the aorta's contraction induced by intracellular calcium, but had no effect induced by extracellular calcium Conclusion: The action of Xueling was similar to the calcium channel's inhibitor verapamil, indicating that its anti hypertension effect was related to the blocking of calcium channel
出处
《浙江中医学院学报》
2002年第4期50-52,56,共4页
Journal of Zhejiang College of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No:39470860)
