摘要
利用花粉管通道法,用含抗除草剂基因bar及抗虫基因pin2的质粒pTW-α转化中籼HD357。经用除草剂Basta进行筛选,得到5株抗性苗。对其进行初步PCR检测,结果表明,有4株PCR结果呈阳性。用PINⅡ基因探针对其进行Southern杂交分析,有2株表现出有杂交信号,说明外源基因已整合转入到水稻基因组中,抗虫性实验尚待继续进行。
Via pollen - tube pathway the indica rice HD - 357 was transformed with plasmid pTW - α containing Bar gene and PIN Ⅱgene. Among five herbicide - resistant plants, four of them can generate 408 bp bands with Bar primers by PCR test, two of them got hybridization bands in the Southern blot hybridization analysis by using 1.5 kb PIN Ⅱ probe. These results indicates that the foreign gene had been transformed into plants genome. The insect resistance bioassay will be continued in the future.
出处
《湖北大学学报（自然科学版）》
CAS
2002年第3期259-262,共4页
Journal of Hubei University：Natural Science
