摘要
本文建立了基于计时电流法的碳纳米管修饰阵列电极同时检测抗坏血酸和尿酸的方法。首先制作了由两个碳工作电极(1mm×2mm)、一个大面积碳对极(2mm×13mm)和一个厚膜Ag/AgCl参比电极构成的集成化碳阵列电极系统,然后以多壁碳纳米管修饰碳阵列电极,最后分别在CH1道电极电位为0.211V测定了抗坏血酸,在CH2道电极电位为0.428V测定了尿酸。实验结果表明,通过聚乙烯不干胶掩膜模板和手工丝网印刷碳技术制作的阵列电极电极面积一致,电活性好。CH1道氧化峰电流(I_(pCH1))与抗坏血酸浓度在4.0×10^(-6) mol/L～4.0×10^(-5)mol/L呈良好的线性关系,CH2道氧化峰电流(I_(pCH2))尿酸浓度在4.0×10^(-7) mol/L～4.0×10^(-6) mol/L呈良好的线性关系。本文设计的电极和建立的测定方法在多种生物样品的同时测定方面具有潜在的价值。
The simultaneous determination of ascorbic acid and uric acid by chronoamperometry based on carbon nanotubes modified carbon electrode array was described in this study. Firstly, the integrated carbon electrode array system was manufactured, including two carbon working electrode（1 mm×2 mm）, a large area of carbon on the anode（2 mm×13 mm） and a thick film of Ag/AgCl reference electrode. Then, the carbon electrode array was modified by carbon nanotubes（MWCNT）. Lastly, the determination of the ascorbic acid in CH1 at 0.211 V and uric acid in CH2 at 0.428 V was developed by chronoamperometry（CA） method. The experiment result showed that the integrated carbon electrode array system through the adhesive carbon mask template and manual screen printing technology has a good performance in simultaneously detecting ascorbic acid and uric acid. The I_（pCH1）（anodic peak current of CH1） was direct proportion to the concentration of ascorbic acid over range from 4.0×10^（-6） mol/L ～4.0×10^（-5） mol/L while I_（pCH2）（anodic peak current of CH2） was direct proportion to the concentration of uric acid over range from 4.0×10^（-7） mol/L ～4.0×10^（-6） mol/L. This work demonstrates that the MWCNT-modified multielectrode array incorporating chronoamperometry is a promising strategy for simultaneous electrochemical determination of biological sample components.
作者
邹蕊
Zou Rui(School of Chemistry and Chemical Engieering Ningxia Normal University, Ningxia 756000, China)
出处
《广东化工》
CAS
2018年第19期33-35,共3页
Guangdong Chemical Industry
基金
宁夏师范学院科研一般项目:NXSFYB1865
关键词
阵列电极
碳纳米管
抗坏血酸
尿酸
multielectrode array
carbon nanotubes
ascorbic acid
uric acid
