摘要
目的研究西黄丸含药血清对人体大肠癌细胞干性标志物表达的影响,初步探讨其作用机制,为西黄丸的临床应用提供新的理论依据。方法以人大肠癌LoVo细胞为研究对象,流式细胞分析术检测不同浓度西黄丸含药血清作用于人体大肠癌LoVo细胞后,CD44和Ep CAM双阳性的细胞数量占大肠癌LoVo细胞数量的比例变化。结果西黄丸能够降低大肠癌肿瘤细胞干性标志物CD44^+/EpCAM^(high)的表达百分比。结论西黄丸对大肠癌肿瘤干细胞活性存在抑制作用,这可能是西黄丸防治大肠癌的重要机制之一。
Objective To study the effect of Xihuang pill containing serum on the expression of stem cell markers in colorectal cancer cells, and to explore its mechanism of action, and to provide a new theoretical basis for the clinical application of Xihuang pill. Methods Taking colon cancer LoVo cells as the research object, the proportion of CD44 and EpCAM double positive cells to the proportion of colorectal cancer LoVo cells was detected by flow cytometric analysis after different concentrations of Xihuang pill containing serum in human colon cancer LoVo cells. Results Xihuang pill can reduce the percentage of CD44＋/EpCAM high expression of colorectal tumor cell stem cells. Conclusion Xihuang pill has inhibitory effect on the activity of colorectal cancer stem cells, which may be one of the important mechanisms of Xihuang pill in preventing colorectal cancer.
作者
孔婧妍
杨帆
KONG Jingyan;YANG Fan(College of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)
出处
《中国中医药现代远程教育》
2018年第20期95-96,117,共3页
Chinese Medicine Modern Distance Education of China
基金
国家自然基金项目【No.81403301】
关键词
大肠癌
西黄丸
含药血清
colorectal cancer
Xihuang pills
drug serum
