摘要
目的：建立乳香中5种乳香酸的含量测定方法,为评价乳香质量提供依据。方法：采用HPLC测定,Agilent SB-C18色谱柱（4.6 mm×150 mm,5μm）;流动相0.1%磷酸-乙腈梯度洗脱;检测波长210,250 nm;柱温30℃。结果：12个批次乳香中α-乳香酸的质量分数为8.6816.1 mg.g-1,β-乳香酸的质量分数为53.5246.9 mg.g-1,3-乙酰-β-乳香酸的质量分数为38.4192.9 mg.g-1,11-羰基-β-乳香酸的质量分数为4.485.81 mg.g-1,11-羰基-β-乙酰乳香酸的质量分数为32.744.2 mg.g-1,5种乳香酸的总质量分数为146.1500.7 mg.g-1。结论：检测的乳香样品中5种乳香酸成分含量有一定差异。
Objective： To develop an HPLC method for determinating the contents of five boswellic acids in Boswellia serrata.Method：Analysis was performed on a zorbax SB C18 column eluted with acetonitrile-0.1% phosphoric acid in water as mobile phases in gradient elution and the detection wavelengths were 210 nm and 250 nm.Result： The five ingredients were separated well.The content ranges of α-boswellic acid,β-boswellic acid,3-acetyl-β-boswellic acid,11-keto-β-boswellic acid and 11-keto-β-acetyl-boswellic acid were 8.68-16.1,53.5-246.9,38.4-192.9,4.48-5.81,32.7-44.2 mg·g-1,respectively.Conclusion： The contents of five individual boswellic acids were different in 12 batches of B.serrata samples.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第10期1330-1333,共4页
China Journal of Chinese Materia Medica
基金
国家重大科技专项经费(2009ZX09103-314)
