摘要
碱-酶两步法水解铬革屑试验结果显示：在碱用量较少且水解温度较低的条件下，处理铬革屑的水解率比单独使用MgO提高62％，比碱-酶-步法提高30％。在两步法中，每次加酶前将浴液的pH值调至酶的最适pH有助于酶活力的充分释放。碱-酶两步法试验中，碱性蛋白酶D处理铬革屑的水解率最高，达到了88．29％，水解液的铬含量为242mg／kg。酸-酶两步法水解铬革屑试验结果显示：草酸预处理后再分别用不同的酶分次水解铬革屑的水解率均在40％-50％，低于碱-酶两步法；水解液的铬含量在14107-16689mg／kg，远高于碱-酶两步法。碱-酶两步法结合处理明显优于酸-酶两步法。
Experiments of chrome leather scraps hydrolyzed by alkaline-enzymatic two-step method show that the hydrolysis rate is 62% higher than that of MgO used only under conditions of using less dosage alkali and lower tem- perature, and 30% higher than that of one-step. It is conducive to release enzymatic activities efficiently by adjusting pH value to optimum in the two-step methocl. The two-step experimental results also indicate that the alkaline prote- ase D has the highest hydrolysis rate which is up to 88.29%, and the content of chromium in hydrolysate is 242mg/ kg. Experiment of chrome leather scraps hydrolyzed by acid-enzymatic two-step method shows that the hydrolysis rate of the given enzymes are all 40% %-50%, which is much lower than that of two-step alkaline-enzymatic method. The contents of chromium in hydrolysate are in the range of 14 107 - 16 689mg/kg, which is much higher than that of alkaline-enzymatic method. Obviously, two-step alkaline-enzymatic method is better than two-step acid-enzymatic method.
作者
刘晓文
宋勇峰
张东方
刘彦
LIU Xiaowen;SONG Yongfeng;ZHANG Dongfang;LIU Yan(The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education,Sichuan University,Chengdu 610065,China;National Engineering Laboratory for Clean Technology of Leather Manufacture,Sichuan University,Chengdu 610065,China)
出处
《中国皮革》
CAS
2018年第10期41-47,共7页
China Leather
关键词
铬革屑
水解
碱-酶法
酸-酶法
氧化镁
草酸
chrome leather scraps
hydrolysis
alkaline-enzymatic method
acid-enzymatic method
magnesiumoxide
oxalic acid
