摘要
目的研究Annexin A2蛋白在人体正常宫颈组织及宫颈鳞状细胞癌组织中的表达差异,及人宫颈鳞状上皮永生化细胞株(H8)和人宫颈鳞癌细胞株(Caski)的表达以及临床意义。方法通过免疫组织化学、Western blot法检测人体正常宫颈组织及宫颈鳞状细胞癌中Annexin A2的表达差异,并体外培养人宫颈鳞状上皮永生化细胞株(H8)和人宫颈鳞癌细胞株(Caski),检测Annexin A2的表达差异。结果 Annexin A2免疫阳性表达产物在人宫颈鳞状细胞癌中其表达量比较高,呈细颗粒状,主要表达于胞浆和胞膜;Annexin A2蛋白在人宫颈鳞状细胞癌组织中表达上调,高于正常宫颈组织(P<0.05)。Annexin A2蛋白免疫阳性表达产物在人宫颈鳞癌细胞株(Caski)表达量较高,主要位于胞浆,淡黄色细小颗粒,分布均匀;Annexin A2蛋白在人宫颈鳞癌细胞株(Caski)中的表达高于人宫颈鳞状上皮永生化细胞株(H8),差异有统计学意义(P<0.05)。结论体内、体外实验结果均显示Annexin A2蛋白在肿瘤组织及细胞中的表达是上调的,可能成为临床研究新的诊断与治疗标记靶点。
Objective To study the different expressions and clinical values of Annexin A2 in human normal uterinecervical tissues, squamous cell carcinoma tissues and the expressions in Caski and H8 cells. Methods Immunohistochemistryand Western blot were used to detect the differences of Annexin A2 protein in human normal uterine cervical tissues andsquamous cell carcinoma tissues. Human cervical cancer cells（Caski） and immortalized human cervical squamous（H8） cellswere cultured. ICC and Western blot were used to detect the expressions of Annexin A2 of them. Results Theimmunostaining product of Annexin A2 was fine-grained and mainly expressed in the cytoplasm and membrane, and itsexpression was higher in cervical squamous cell carcinoma. The Annexin A2 expression in the tumor tissue was significantlyhigher than that of the normal tissues（P〈0.05）. The Annexin A2 protein immunoreactive products were located mainly in thecytoplasm, light yellow fine particles, and distributed evenly. The expression level was higher in human cervical squamous cellcarcinoma cell line（Caski）. Compared with H8 cells, the Annexin A2 expression was significantly higher than that in Caskicells（P〈0.05）. Conclusions The Annexin A2 protein is much higher in human uterine cervical squamous cell carcinomatissues and in human cervical cancer cells（Caski）. It might be related to the genesis and development of the human uterinecervical squamous carcinoma and it might be a new valuable tumor marker.
作者
陈雪
周星
冀雅铭
陈红
张金库
CHEN Xue;ZHOU Xing;JI Yaming;CHEN Hong;ZHANG Jinku(Pathology Department of Baoding First Central Hospital,Baoding,Hebei 071000,China)
出处
《中国热带医学》
CAS
2018年第9期870-872,888,共4页
China Tropical Medicine
