摘要
采用聚合酶链反应－单链构象多态性分析（ＰＣＲ－ＳＳＣＰ）、多重引物ＰＣＲ和巢式引物ＰＣＲ，对同一宫颈癌组织中ｐ５３基因（第６～８外显子）突变以及人乳头状瘤病毒（ＨＰＶ）和人巨细胞病毒（ＨＣＭＶ）感染进行相关性研究。并与正常宫颈组织进行对照。结果：３８例宫颈癌组中，２例有ｐ５３第７外显子突变。其中１例伴有ＨＰＶ１６感染，２例伴有ＨＣＭＶ感染。宫颈癌组ＨＰＶＩ６、１８ＤＮＡ的阳性率为６３．２％（２４／３８），ＨＣＭＶＤＮＡ为８４．２％（３２／３８）。２１例对照组中，ＨＰＶ１６、１８ＤＮＡ和ＨＣＭＶＤＮＡ的阳性率分别为４．８％和３８．１％（Ｐ＜０．００５）。ＨＰＶ１６、１８阳性的子宫颈癌中，８７．５％伴有ＨＣＭＶ感染。对照组中，无一例同时检测出ＨＰＶ１６、１８和ＨＣＭＶ者。提示：宫颈癌组织中，ｐ５３基因突变并不常见，其突变与ＨＰＶ１６、１８感染无显著关系。宫颈癌与ＨＰＶ１６、１８关系密切，ＨＣＭＶ可能与ＨＰＶ协同作用，导致宫颈癌的发生。
bjective To investigate the role of hurnan papillomavirus (HPV). human cytomegalovirus (HCMV) infections and p53 gene mutations in the oncogenesis of cervical cancer and to clarify the asso- ciation between p53 inactivation and the presence of HPV DNA. Methods We examined 38 primary cervical carcinomas and 21 normal cervical specimens for the presence of HPV and HCMV DNA sequences by multiple primers PCR and nest primers PCR, The structure of p53 gene (exons 6-8) was also analyzed by PCR-SSCP silver staining method. Results Mutations of p53 gene (exon 7) were detected in 2 of 38 tumors, One of the cases with p53 mutation was positive for HPV 16 and two positive for HCMV. HPV 16 and 18 infections were not- ed in 63.2% (24/38) of the tumors, the postitive rate of HCMV was 84.2 % (32/38) . However, HPV 16, 18 and HCMV infection occurred in4.8 % and 38.1% respectively in 21 normal cervical specimens. 21 of the 24 HPV 16, 18 positive tumors were also HCMV positive, but none of the norrnal cervicaI tis- sues was infected with both NPV and HCMV. Conclusion Detection of p53 mutations in cervical carcinoma is infrequent and apparently indepen- dent of HPV intection, Cervical carcinoma is strongly associated with HPV 16 and 18 infection. A syn- ergistic interaction may occur between HPV and HCMV infections in the oncogenesis of cervical cancer.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1995年第11期654-657,共4页
Chinese Journal of Obstetrics and Gynecology
基金
美国中华医学基金会
国家自然科学基金
