摘要
目的探究甘草黄酮(Glabridin,Gla)对LPS致小鼠急性炎症的影响及其潜在机制的研究。方法将Gla(80 mg/kg)连续给予小鼠灌胃3 d,每天1次。于末次给药2 h后,经腹腔注射LPS(30 mg/kg)建立小鼠急性炎症模型。获取小鼠血清进行ALT和AST的活力分析;研磨肝组织并采用PCR法检测TNF-α、IL-1β、i NOS和COX-2等炎症因子的m RNA表达变化;制作肝组织冰冻切片,通过H&E染色观察肝脏的病理形态学改变。结果 Gla能够降低AST和ALT的活性水平,减少肝组织中TNF-α、IL-1β、i NOS和COX-2等炎症因子的m RNA表达,改善肝脏病理组织学损害。结论 Gla能够通过减少肝组织炎症因子的释放对LPS诱导的急性肝炎产生保护作用。
Objective To investigate the effects of glabridin（Gla） on lipopolysaccharide（LPS）-induced acute inflammation in mice, and explore the underlying mechanism. Methods Mice are intragastrically administered with Gla at the doses of 80 mg/kg during three continuous days. Two hours after the last administration of Gla, mice are then given an intraperitoneal injection of LPS（30 mg/kg） to build a murine model of acute inflammation process. Serum levels of AST and ALT, m RNA expressions of TNF-α, IL-1β, i NOS, and COX-2 in the hepatic tissue, and the pathomorphological changes in liver are evaluated with the recommended ELISA protocols, PCR assays, and H＆E staining method, respectively. Results Gla could markedly inhibit the levels of AST and ALT in serum, decrease the m RNA expressions of these proinflammatory factors including TNF-α, IL-1β, i NOS and COX-2 in live tissues, and improve the morphological changes of liver from mice with LPS-induced acute inflammation reaction. Conclusion Collectively, these findings reveal that Gla might exert the protective effects on LPS-induced acute inflammation via suppression of inflammatory mediators in mice.
作者
李冰
杨薇娜
杨广德
LI Bing;YANG Wei-na;YANG Guang-de(School of Pharmacy,Xi＇an Jiaotong University,Xi＇an 710061,China;Xi＇an Medical University,Xi＇an 710021,China)
出处
《现代中药研究与实践》
CAS
2018年第4期14-17,21,共5页
Research and Practice on Chinese Medicines
基金
国家自然科学基金项目(81373368)
关键词
脂多糖
甘草黄酮
炎症因子
肝损伤
Lipopolysaccharide
glabridin
inflammatory factor
acute hepatic injury
