摘要
目的探讨环氧合酶2(COX-2)在酒精性肝病(ALD)发病机制中的作用。方法用酒精灌胃建立大鼠酒精性肝病模型,分别于4、8、12和16周取肝组织,用生化比色法检测肝匀浆6-酮-前列腺素F1α(6-K-PGF1α)、血栓素B2(TXB2)、甘油三酯(TG)、氧自由基(ORF)、抗超氧阴离子(ASOA)、超氧化物歧化酶(SOD)及丙二醛(MDA)含量;用HE、苏丹Ⅳ和天狼猩红染色观察肝组织病理形态;RT-PCR法观察肝组织COX-2 mRNA表达。结果4、8、12和16周的大鼠肝组织COX-2 mRNA相对表达量分别为正常对照组的1.29、1.70、1.92、3.38倍,除4周外P<0.01;COX-2蛋白表达逐渐增强;肝组织COX-2 mRNA相对表达量与肝匀浆OFR、MDA、TG、和TXB2呈正相关(P<0.01);与ASOA、SOD、6-K-PGF1α呈负相关(P<0.01)。结论COX-2与酒精所致大鼠肝脏病变密切相关,是ALD发病过程中重要因素之一。结论COX-2与氧化应激密切相关,二者在ALD发病中发挥重要作用。
Objective To explore the effect of cyclooxygenase 2 （COX-2） in the pathogenesis of alcoholic liver disease （ALD）. Methods A model of ALD was developed with Wistar rats by intragastric alcohol. Liver samples were collected by the end of 4th, 8th, 12th and 16th week respectively. The concentrations of triglyceride（ TG）, oxygen free radical （ORF） , superoxide dismutase （SOD） , anti-superoxide anion （ASOA） , 6-ketone-prostaglandin F1α （6-K-PGF1α） , thromboxane B2 （ TXB2 ） and malondiadehyde （ MDA ） in the liver homogenate were measured by biochemistry chromatometry. Pathology of hepatic tissue was examined by hematoxylin eosin, sudan Ⅳ and sirius red staining. The protein and mRNA expression of COX-2 in liver tissues were examined by immunohistochemical method and reverse transcription-polymerase chain raction （RT-PCR） respectively. Results By the end of 4th,8th, 12th and 16th week, the content of COX-2 mRNA in liver tissue of model rats were 1.29,1.70,1.92 and 3.38 times the same as normal rats（P 〈0.01 ,except for 4th week） ,and the expression of COX-2 protein increased also. Pearson correlation analysis showed that the COX-2 mRNA expression was correlated positively with the content of OFR, MDA,TG, and TXB2 （P 〈 0. 01 ） , and correlated negatively with the content of ASOA, SOD and 6-K-PGF1αin the liver homogenate （P 〈 0. 01 ）. Conclusion There is a close correlation between COX-2 and oxidative stress, which plays an important role in the etiopathegenesis of ALD.
出处
《基础医学与临床》
CSCD
北大核心
2007年第1期68-71,共4页
Basic and Clinical Medicine
关键词
酒精性肝病
环氧合酶-2
氧化应激
alcoholic liver disease
cyclooxygenase 2
oxidative stress
