摘要
目的研究白藜芦醇(resveratrol,Res)对酒精性肝损伤后肝组织氧化反应和炎性反应的影响及与一氧化氮(NO)、诱导型一氧化氮合成酶(iNOS)通路的关系。方法大鼠灌胃给予55度红星二锅头复制肝损伤模型。大鼠随机分为正常对照组、肝损伤模型对照组、Res低剂量组(25 mg.kg-1)、Res中剂量组(50 mg.kg-1)、Res高剂量组(100 mg.kg-1),每日2次,连续7 d。采用试剂盒测定肝脏组织乳酸脱氢酶(LDH)、活性氧(ROS)、还原型谷胱甘肽(GSH)、NO、iNOS和血清IL-10、IFN-γ水平。Western blot测定iNOS表达,RT-PCR测定iNOS mRNA表达。结果与正常对照组相比,酒精性肝损伤模型组肝组织LDH、ROS、NO浓度显著升高(P<0.01)、GSH显著下降(P<0.01),血清IFN-γ水平显著升高、IL-10显著降低(P<0.01),肝组织iNOS和iNOS mRNA表达增加(P<0.01)。与模型组相比,Res显著降低肝脏组织ROS、LDH、NO浓度(P<0.01),降低血清IFN-γ水平和升高血清IL-10与肝脏组织GSH含量(P<0.01),减少iNOS和iNOS mRNA表达(P<0.01)。结论 Res可能通过NO通路,消除氧化性应激状态,改变炎性因子水平,对酒精性肝损伤发挥防治作用。
OBJCETIVE To investigate the effects of resveratrol（Res） on alcohol-induced hepatic injury and the relationship with nitric oxide（NO） pathway.METHODS The rats were randomly divided into 5 groups,including the normal control group,alcohol-induced hapatic injuried model group,Res 25 mg.kg-1 group,Res 50 mg.kg-1 group and Res 100 mg.kg-1 group.The hepatic injuried model was imitated by ig 55° Red Star Erguotou.Res was administrated twice daily,continuously for 7 days.The contents of lactate dehydrogenase（LDH）,reactive oxygen species（ROS）,reduced glutathione（GSH）,nitric oxide（NO）,induced nitric oxide synthase（iNOS） in liver and the concentrations of serum IFN-γ,IL-10 were determined by the commercial kits.The expression of iNOS was determined by Western blot and the level of iNOS mRNA was measured by real-time RT-PCR.RESULTS Compared with the normal control group,the concentrations of LDH,ROS,NO in liver were significantly increased（P0.01） and GSH was significantly reduced（P0.01） in the model group.Meantime,in the model group,the level of serum IFN-γ was significantly augmented and serum IL-10 depressed（P0.01）.In addition,the expressions of iNOS and iNOS mRNA were significantly increased（P0.01）.Res reduced the levels of LDH,ROS,NO and iNOS in liver and IFN-γ in serum compared with the model group,respectively.Furthermore,Res significantly increased the concentrations of serum IL-10 and hepatic GSH.Additionally,Res significantly suppressed the expressions of iNOS and iNOS mRNA in liver.CONCLUSION Res may prevent liver from alcoholic impairment via NO pathway.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2010年第9期772-777,共6页
Chinese Journal of Modern Applied Pharmacy
