摘要
试验旨在探讨丙氨酰-谷氨酰胺(Ala-Gln)和精氨酸(Arg)对乌鳢生长与免疫功能的影响。在乌鳢配合饲料中添加不同比例Ala-Gln和Arg,配成7种等氮(42.5%粗蛋白质)、等能(18.5 MJ/kg)饲料,以前期试验得到乌鳢生长最适需求量的Ala-Gln和Arg添加量(5.0 g/kg和20 g/kg)为100%,在此基础上等比例添加0%、20%、40%、60%、80%、100%、120%。饲喂初始体质量为(9.56±0.24)g乌鳢8周。结果表明:饲料中Ala-Gln和Arg添加量的比例在20%~60%时,乌鳢的增重率随着添加比例的增加而不断升高,60%添加组的蛋白质效率和饲料效率均显著高于对照组(P<0.05);血清和肝脏谷胱甘肽过氧化物酶(GSH-Px)随Ala-Gln和Arg的添加而逐渐升高,120%添加组的血清GSH-Px显著高于20%~60%组和对照组(P<0.05);头肾和脾脏的溶菌酶(LZM)活性都随Ala-Gln和Arg的添加呈现先升高后降低或平稳的趋势,当添加量为40%~60%时,头肾的LZM活性显著高于对照组和20%的添加组(P<0.05);白细胞介素1β(IL-1β)随Ala-Gln和Arg的增加而降低,60%添加组的IL-1β显著低于对照组、20%和40%添加组(P<0.05),80%的添加组的IL-2显著高于对照组、20%~60%添加组(P<0.05)。攻毒存活率随Ala-Gln和Arg的增加而升高,60%添加组的攻毒存活率显著高于对照组、20%~40%添加组(P<0.05)。在本试验条件下,饲料中添加60%~68.76%Ala-Gln和Arg可以有效促进乌鳢生长、饲料利用和提高抗氧化、免疫保护的能力,以增重率和蛋白质效率为指标,经单因素方差分析和抛物线回归分析得出乌鳢饲料中Ala-Gln和Arg的适宜水平为3.0%~3.4%、12.0%~13.75%。
To evaluate the influence of propylamine acyl-glutamine （Ala-Gln） and Arginine （Arg） on growth and immunity of Channa argus. Different proportions of Ala-Gln and Arg were added to the L77anna argus compound feed to compose 7 kinds of isonitrogen （42.5% crude protein） and isopotential （18.5 MJ/kg） diets, and the addition of Ala-Gln and Arg to the optimum growth of Channa argus was added. 100% of the addition group, based on this increase or decrease in the proportion of 0%, 20%, 40%, 60%, 80%, 100%, 120%. Feeding the initial weight （9.56 _＋ 0.24） g of Channa argus for 8 weeks. The results showed that when the propor- tion of Ala-Gln and Arg added in feed was between 20% and 60%, the growth rate of Cbanna argus increased significantly with the increase of the ratio （P〈0.05）. The protein efficiency and the feed efficiency of the 60% added group was significantly higher than that of the control group （P〈0.05）. The GSH-Px of serum and liver were increasing significantly with the addition of Ala-Gln and Arg. The GSH-Px was significantly higher in the 120% group than in other groups （P〈0.05）. The activity of lysozyme in head kidney and spleen increased first, then decreased or remained stable with the addition of Ala-Gln and Arg. When added at 40%, the LZM activity of head kidney was sig- nificantly higher than that of the control group and 20% addition group （P〈0.05）; IL-115 decreased with the increase of Ala-Gln and Arg, 60% of the added group was significantly lower than the control group, 20% and 40% addition group （P〈0.05）, the IL-2 level in the 80% added group was significantly higher than that in the control group and 20%-60% addition group （P〈0.05）. In this experimental conditions, adding 60%-68.76% of Ala-Gln and Arg to the feed can effectively improve growth performance, feed utilization and muscle protein synthesis of Channa argus, Based on the average weight gain rate and protein efficiency with regression equation analyses, the suitable level of Ala-Gln and Arg in feed of Channa argus were 3.0%-3.4% and 12.0%-13.75%.
作者
孔祎頔
郑伟
陈秀梅
刘洪健
单晓枫
陈玉珂
王桂芹
Kong Yi＇di;Zheng Wei;Chen Xiumei;Liu Hongjian;Shan Xiaofeng;Chen Yuke;Wang Guiqin
出处
《饲料工业》
北大核心
2018年第16期6-11,共6页
Feed Industry
基金
国家现代农业(特色淡水鱼)产业技术体系建设专项资金资助[CARS-46]
国家自然科学基金项目[31372540]
