摘要
以文冠果蛋白为研究对象,采用酶解法制备文冠果蛋白酶解肽,并对其体外抗高血压活性进行研究。以水解度为指标确定酶解条件。蛋白水解物经超滤得到分子量为<1k Da,1~5 k Da和>5 k Da 3个区段的多肽组分,测定其对血管紧张素转化酶(Angiotensin Converting Enzyme,ACE)的抑制率。最佳酶解条件为:底物质量浓度40g/L,加酶量8%(质量分数)(碱性蛋白酶),酶解时间175 min,酶解温度55℃和酶解p H 9.0,此条件下水解度最高为22.97%;3种多肽组分中<1k Da的ACE抑制率最高为83.43%,其半抑制浓度(50%inhibitory concentration,IC50)为0.394 mg/m L。文冠果蛋白酶解多肽具有显著的体外抗ACE活性,可作为降血压肽的开发原料。
To prepare Xanthoceras sorbifolia peptide by enzymatic hydrolysis and study its anti-hypertensive activity in vitro. The hydrolysis conditions were determined by degree of hydrolysis index. Protein hydrolysates were ultrafiltered to obtain three peptide fractions with molecular weights of 〈 1kDa, 1-5kDa and〉 5kDa, and their inhibition rate on （Angiotensin Converting Enzyme, ACE） was determined. The optimum enzymatic conditions were as follow： the substrate mass concentration was 40 g/L, the enzyme dosage was 8% （alkaline protease）, the enzymatic time was 175 min, the enzymatic temperature was 55℃ and the enzymatic pH was 9.0, and with this condition, the highest degree of hydrolysis was 22.97%.ACE inhibition rate of 〈 1kDa peptide fraction was 83.43% （IC 50 =0.394mg/mL）, which was the highest among three peptide fractions. The proteolytic peptides of Xanthoceras sorbifolia bunge have significant anti-ACE activity in vitro and can be used as a raw material for the development of anti-hypertensive peptides.
作者
张齐
阎怡竹
刘清清
李聪
申烨华
邓建军
ZHANG Qi;YAN Yizhu;LIU Qingqing;LI Cong;SHEN Yehua;DENG Jianjun(College of Food Science and Engineering,Northwest University,Xi′an 710069,China;School of Chemical Engineering,Northwest University,Xi′an 710069,China;College of Chemistry and Materials Science,Northwest University,Xi′an 710069,China)
出处
《西北大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第4期551-556,共6页
Journal of Northwest University（Natural Science Edition）
基金
国家自然科学基金资助项目(21476184)
关键词
文冠果
降血压肽
水解度
聚丙烯酰胺凝胶电泳
Xanthoceras Sorbifolia B.
anti-hypertensive
degree of hydrolysis
SDS-polyacrylamide gel electrophoresis
