摘要
目的 :研究被动吸烟对帕金森病 (PD)大鼠的影响 ,探讨其作用机制。方法 :通过 6—羟多巴胺 (6 OH DA)脑立体定向注射术建立大鼠帕金森病模型。采用生化、免疫组织化学的方法观察PD大鼠纹状体脑胶质细胞源性神经营养因子表达 (GDNF)及多巴胺 (DA)含量的变化以及术前 4周开始给予被动吸烟 (持续 6周 )和术后 3周给予的被动吸烟持续 2周对上述指标的影响。结果 :术前及术后吸烟的PD大鼠纹状体DA含量、脑胶质源性神经营养因子表达较PD组有明显改善 (P <0 0 5 )。结论
Objective:To investigate the effects of passive smoking on the PD in rats.Methods:6 OHDA was stereoscopically injected into right side of the mesencephic ventral tegmental area(VTA) and substantia nigra pars compacta(NpcS). Using biochemical, immunohistochemical techniques, We observed the effects of passive smoking beginning four weeks before the operation or three weeks after the operation in the successful models on dopamine content and GDNF expression in striatum.Results:In rats receiving passive smoking beginning four weeks before the operation or three weeks after the operation in the successful models, DA content and GDNF expression were increased compared to the PD group(P<0 05).Conclusion:passive smoking can protect DA neurons of substantia nigra from the damage of 6 OHDA.
出处
《河南实用神经疾病杂志》
2002年第6期8-10,共3页
Henan Journal of Practical Nervous Diseases
