摘要
目的：研究丹皮酚对肝癌原位移植瘤组织凋亡及半胱氨酸天冬氨酸蛋白酶-9（Caspase-9）、核转录因子NF-κB p65的影响。方法：用MHCC-97H人肝癌细胞建立裸鼠肝癌原位移植瘤,之后将50只造模成功裸鼠随机分入生理盐水组、5-氟尿嘧啶组、丹皮酚低剂量组、中剂量组、高剂量组,分别腹腔注射生理盐水、5-氟尿嘧啶、丹皮酚20 d后,取瘤体组织。免疫组化法原位检测组织细胞凋亡及Caspase-9、NF-κB p65的表达,并结合Image-Pro Plus 6.0图像处理系统分析免疫组化结果。结果：与生理盐水组相比,丹皮酚低、中、高剂量组及氟尿嘧啶组凋亡程度均有不同程度升高,差异有统计学意义（P〈0.05）,丹皮酚高剂量组凋亡最显著。丹皮酚低、中、高剂量组和氟尿嘧啶组Caspase-9阳性表达强于生理盐水组,NF-κB p65表达弱于生理盐水组。丹皮酚中剂量组Caspase-9、NF-κB p65的表达与氟尿嘧啶组无明显差异（P〉0.05）。结论：丹皮酚可以诱导裸鼠体内肝癌细胞凋亡,其作用机制可能是通过上调Caspase-9的表达,同时抑制NF-κB p65而实现。
Objective：To investigate the effect of paeonal on apoptosis and the expression of NF-κB p65,Caspase-9 transplanted tumor of human hepatocellular carcinoma in nude mice. Methods：We apply MHCC-97 human liver cancer cells to establish nude mouse orthotopic transplantation of liver cancer tumor,since then 50 building successful nude mice were randomly drawn in blank model group,the 5-fluorouracil group,paeonol low dose group,middle dose group,high dose group,and were intraperitoneal injected with Paeonal,5-fluorouracil,for 20 days. The apoptosis and the expression of NF-κB p65,Caspase-9 were tested by immunohistochemistry,connecting with the Image-Pro Plus 6.0. Results：Compared with normal saline group,the apoptotic degree of paeonol low,medium and high dose group and fluorouracil group were significantly increased（P〈0.05）,the apoptotic expressed strongest. The positive expression of Caspase-9 in paeonal low,medium and high dose group and fluorouracil group were stronger than that in saline group,and NF-κB p65 expression was weaker than that of saline group. There was no significant difference in the expression of Caspase-9 and NF-κB p65 between the paeonol low dose groups and the fluorouracil group（P〈0.05）. Conclusion：Paeonal can induce liver cancer cell apoptosis in nude mice and its mechanism may be through the increase the expression of Caspase-9 and inhibit the expression of NF-κB p65.
作者
杨春
张春虎
尚娇
曾伟
梁慧
李云辉
YANG Chun;ZHANG Chunhu;SHANG Jiao;ZENG Wei;LIANG Hui;LI Yunhui(Affiliated Tumor Hospital of Xiangya Medical College of Central South University, Changsha 410013, Hunan, China;Xiangya Hospital of Central South University, Changsha 410008, Hunan, China)
出处
《辽宁中医药大学学报》
CAS
2018年第6期21-24,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
湖南省中医药管理局项目(2009046)
