摘要
目的探讨丹皮酚对人结肠癌LoVo细胞生长及凋亡的作用。方法用丹皮酚作用于体外培养的人结肠癌LoVo细胞,采用MTT(四甲基偶氮唑蓝)比色法,分析其对LoVo细胞生长的抑制作用,采用流式细胞仪测定细胞凋亡率,光镜下观察细胞形态。结果丹皮酚在(7.81～250)mg/L质量浓度范围内呈时间、剂量依赖方式抑制结肠癌LoVo细胞生长(P<0.05或P<0.01),丹皮酚在(15.63～250)mg/L质量浓度范围内作用48h后,光镜下可见LoVo细胞呈典型的凋亡形态学改变;流式细胞仪检测出现凋亡峰,其测得的细胞凋亡率与对照组比较,有非常显著性差异(P<0.01)。结论丹皮酚对人结肠癌LoVo细胞生长具有抑制作用,其机制与诱导肿瘤细胞凋亡有关。
Objective To investigate the effect of paeonol on growth and apoptosis of human colon carcinoma cell line LoVo.Methods In vitro cultured human colon carcinoma cell line LoVo was treated with paeonol.Cell proliferation was evaluated by MTT.Cell apoptosis rate was determined by flow cytometry,and apoptosis was confirmed by morphology.Results The proliferation of LoVo was inhibited significantly by paeonol in a dosage dependant manner（range from7.81～250 mg/L,P〈0.05 or P〈0.01）.Observing under microscope,some LoVo cells underwent a typical apoptosis after incubation with paeonol for 48h.Cells apoptosis rate was significantly increased in paeonol group compared with that in the controls（P〈0.01）.Conclusion The results indicated that paeonol inhibits the proliferation of colon carcinoma LoVo cells.The anticancer mechanism is related to induce cells apoptosis.
出处
《实用癌症杂志》
2008年第6期557-560,共4页
The Practical Journal of Cancer
基金
江西省教育厅资助课题(NOS00194)
