摘要
为探明山葡萄叶片低温伤害的生理机制,以抗寒性差异明显的‘双丰’(山葡萄种内杂交种)和‘左优红’(种间杂交种)为试材,对低温处理过程中叶绿素荧光参数和活性氧代谢的相关指标进行测定。结果表明,随低温胁迫加剧,两个品种叶片的叶绿素含量、Chl.a/b、Fv/Fm值均呈显著下降趋势,1–qp值及MDA含量明显增加;整个胁迫过程中NPQ值较稳定的‘双丰’其叶片遭受低温伤害程度较轻,NPQ值一直下降的‘左优红’叶片伤害程度较重,‘双丰’的NPQ值始终显著高于‘左优红’。‘双丰’叶片的SOD和APX活性始终较‘左优红’高。说明低温使山葡萄叶片叶绿素分解,PSⅡ电子传递受阻,激发能增加,造成叶绿体膜脂过氧化损伤;热耗散及抗氧化酶共同作用减轻了活性氧对叶片PSⅡ的伤害程度。由此可见,热耗散NPQ值及活性氧清除酶SOD和APX活性的差异可能是造成两个山葡萄品种叶片低温伤害程度不同的重要原因。
The parameters of chlorophyll fluorescence and reactive oxygen metabolism were measured in two different cold-tolerant amur grape cultivars of‘Shuangfeng＇（intraspecific hybridization）and‘Zuoyouhong＇（interspecific hybridization）. The chlorophyll content,Chl.a/b and Fv/Fm values of the two cultivars were significantly decreased,while the value of 1–qp and MDA content increased significantly with the temperature decreasing gradually. Stable NPQ value and the higher SOD and APX activities in‘Shuangfeng＇leaf showed the lower damage degree than‘Zuoyouhong＇. The results suggested that the leaf chlorophyll decomposition,electron transfer blocked of PSⅡand excitation energy increased under the low temperature stress eventually resulted in the lipid peroxidation of chloroplast membrane. Thermal dissipation and antioxidant enzymes were helpful to reduce the damage of reactive oxygen to PSⅡ. Therefore,the differences in NPQ and the activities of SOD and APX might be the important aspects that caused the genotype differences in cold injury.
作者
赵滢
王振兴
许培磊
杨义明
刘迎雪
刘海双
艾军
ZHAO Ying;WANG Zhenxing;XU Peilei;YANG Yiming;LIU Yingxue;LIU Haishuang;andAI Jun(Institue of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China)
出处
《园艺学报》
CAS
CSCD
北大核心
2018年第4期650-658,共9页
Acta Horticulturae Sinica
基金
国家现代农业产业技术体系建设专项资金项目(CARS-29-7)
中国农业科学院创新工程协同创新项目(CAASXTCX-2016017004)
中央级公益性科研院所基本科研业务费专项(1610342016011)
关键词
山葡萄
低温伤害
叶绿素荧光
活性氧
Vitis amurensis
cold injury
chlorophyll fluorescence
reactive oxygen species
