摘要
目的探讨催产素对创伤后应激障碍（PTSD）样大鼠海马组织丝裂原活化蛋白激酶磷酸酶-1（MKP-1）表达的影响。方法将18只SD大鼠随机分为对照组、模型给药组和模型组各6只。模型给药组和模型组给予国际认定的单次延长应激（SPS）方法刺激建立大鼠PTSD模型，模型给药组在应激后8h内给予催产素持续14d，通过旷场实验和Morris水迷宫观察大鼠的行为学变化。采用实时荧光定量PCR（qRT—PCR）和Western blot方法分别检测大鼠海马组织中MKP-1mRNA和蛋白的表达。结果（1）在旷场实验中，与模型组[（2．50±1．05）次、（22．16±7．14）次]相比，模型给药组[（5．16±1．17）次、（32．83±5．71）次]与对照组[（6．67±2．16）次、（39．83±4．62）次]大鼠的直立次数与进格次数减少（P〈0．05）。在水迷宫实验定位航行中，模型组与模型给药组相比，大鼠的潜伏期延长（P〈0．05），在空间探索实验中，与模型给药组相比，模型组大鼠的潜伏期延长、穿台次数减少（P〈0．05）。（2）与模型给药组（1．30±0．03）相比，模型组（4．04±0．46）大鼠海马组织中的MKP-1mRNA表达明显上调（P〈0．05）；与模型给药组（1．46±0．27）相比，模型组（1．95±0．68）大鼠海马组织中的MKP-1蛋白表达显著增加（p〈0.05）。结论催产素可通过调控MKP-1的表达来保护PTSD大鼠的学习记忆能力以及减少大鼠应激相关表现。
Objective To investigate the effect of oxytocin on the expression of MKP-1 level in the hippocampus of post-traumatic stress disorder rats. Methods Eighteen SD rats were randomly divided into the control group, administration group and the experimental group with 6 rats in each group. The administration group and the experimental group were treated with internationally recognized single prolongation stress （SPS） method to stimulate the rats in order to establish PTSD models. The 14-day oxytocin intervention was given to rats of the administration group after the SPS stimulation within 8 hours. And the behavioral changes of rats were observed by open-field test and Morris water maze test. The changes of MKP-1 mRNA in the hippoeampus of rats were detected by real-time quantitative PCR （qRT-PCR） ,and the levels of MKP-1 Protein in the hippocampus of rats were detected by Western Blot. Results （ 1 ） Compared with the model group （2.50± 1.05 and 22.16±7.14） ,the times of the standing and crossing grid section quantities in the open-field test in the administralion group （ 5.16±1.17and 32.83±5.71 ） and control group （ 6.67±2.16 and 39.83±4.62 ） significantly decreased （/）〈0.05）. Mmxis water maze showed that the incubation of the model group was markedly prolonged （P〈0.05） compared with that of the administration group, while in spatial probe test, the incubation period of the rats in administration group was prolonged, and the number of wearing stage decreased （P〈0.05）. （2） Compared with the administration group （1.30±0.03） , the expression of MKP- 1 mRNA in hippocampus of rats in model group （4.04±0.46） was notably up-regulated （P〈0.05）.And the protein level of MKP-1 in model group（1.95±0.68） was also increased compared with that in administration group （1.46±0.27） （P〈0.05）.Conclusions Oxytocin can protect the learning and memory ability and reduce the stress-related performance of rats via regulating the expression of MKP-1.
作者
邢文龙
王梅子
时若欢
刘超猛
张桂青
Xing Wenlong , Wang Meizi,Shi Ruohuan,Liu Chaomeng,Zhang Guiqing(Medical College, Shihezi University, Shihezi 832003, China; Department of Psychology and Rehabilitation, the First Affiliated Hospital of the Medical College, Shihezi University, Shihezi 832008, Chin)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2018年第3期230-234,共5页
Chinese Journal of Behavioral Medicine and Brain Science
基金
“十二五”新疆生产建设兵团医药卫生重点领域科技攻关项目（2012BA023）
