摘要
目的分析近年辽宁省腹泻病例中检出的肠炎沙门菌血清型、耐药性及分子分型。方法对辽宁地区50株沙门菌临床分离株进行血清型分型;采用微量肉汤法,进行16种抗菌药物的药敏试验;采用脉冲场凝胶电泳(PFGE)方法对选取的16株肠炎沙门菌株进行基因分型。结果肠炎沙门菌是沙门菌中的优势血清型。所有菌都对链霉素耐药。16株肠炎沙门菌分成7种型别,其中6个PFGE型别间仅有1条~3条带的差异,相似度在78.68%~100%,菌株间具有很近的亲缘关系。结论辽宁省感染性腹泻病例中沙门菌感染率较低。PFGE分子分型较多,无明显聚集性。
Objective To analyze the serotype,drug resistance and molecular typing of Salmonella enteritidis detected in diarrhea cases in Liaoning Province in recent years. Methods The clinical isolates of 50 strains of Salmonella in Liaoning were serotyped. The antibiotic susceptibility tests of 16 kinds of antibacterial drugs were carried out by using the micro broth method.By using the pulsed-field gel electrophoresis（ PFGE）,16 Salmonella enteritidis strains were conducted for genotyping.Results Salmonella enteritidis was the dominant serotype in Salmonella. All bacteria were resistant to streptomycin. Sixteen strains of Salmonella enteritidis were divided into seven types,of which there were only differences in one to three bands among the six PFGE types. The similarities ranged from 78. 68% to 100%,and the strains were closely related. Conclusion Salmonella infection rate is low in infectious diarrhea cases in Liaoning Province. PFGE molecular typing is more without significant aggregation.
作者
雷露
毛玲玲
刘学升
王博
孙英伟
姚文清
LEI Lu, MAO Ling - ling, LIU Xue - sheng, WANG Bo, SUN Ying - wei, YAO Wen - qing(Liaoning Center for Disease Control and Prevention, Shenyang, Liaoning 110005, Chin)
出处
《中国卫生检验杂志》
CAS
2018年第5期534-536,共3页
Chinese Journal of Health Laboratory Technology
基金
艾滋病和病毒性肝炎等重大传染病防治(2012ZX1000-4209)
关键词
肠炎沙门菌
耐药性
脉冲场凝胶电泳分子分型
Salmonella enteritidis
Drug resistance
Pulsed -field gel electrophoresis
