摘要
目的了解生鲜肉类中肠炎沙门菌耐药性及基因变异情况。方法药敏试验采用K-B法,用碱裂解法提取细菌的质粒DNA,采用肠杆菌重复基因间隔重复共有序列(ERIC-PCR)技术对10株肠炎沙门菌进行基因分析,运用NTSYS-PC2.0软件进行同源性比较,聚类分析。结果药敏呈现出多重耐药;基因谱系与聚类分析可将菌株分为3大类,扩增片段长度为250bp～2000bp,在85%的同源度上可分为A、B、C等3个型,其中A型2株,B型6株,C型2株。结论生鲜肉中肠炎沙门菌耐药情况比较普遍,10株肠炎沙门菌基因变异较小,ERIC-PCR技术是分析、鉴定肠炎沙门菌,追溯食物中肠炎沙门菌的有用和可行的工具。
Objective To assess the antibiotics susceptibility and genetical diversity of salmonella enteritidis isolated from raw and fresh meats. Methods Gene analysis by antibiotis susceptibility, ERIC-PCR techniquea and pIasimid fingerprinting were put into practice for the detection of gene variation of 10 strains of salmnoella enteritidis. The similarities between different strains were obtained by software NTSYS-PS 2.0 for cluster analysis. Results 10 strains of salmonella enteritidis appear multi-antibiotics resistance. These strains were subtyped into 3 groups A, B, C according to the electrophoresis profiles sinilarity 85 % standard or above, including 2 strains, 6 strains and 2 strains respectively. Conclusion These strains exist wide antibiotics resistance, 10 strains of Salmonella enteritidis have lower genetical diverity. ERIC-PCR is a powefultool for differentiating salmonella enteritdis and tracking the transmission of salmonella enteritidis in food.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第10期1227-1228,共2页
Chinese Journal of Public Health
关键词
肠炎沙门菌
ERIC-PCR
耐药性
聚类分析
salmone enteritidis
ER IC-PCR
antibiotics susceptibility
cluster analysis.
