摘要
测序技术发展到今天,取得了里程碑式的突破,加深了我们对生物多样性、生理代谢及疾病的理解,促进了生物学的发展。二代测序技术是目前最为广泛使用的测序技术,和现有实验手段结合又开拓了许多新的应用领域。RNA-seq就是二代测序技术的最主要的应用之一。它不仅完善了基因组的基因注释,发现新的可变剪切位点及新的基因,也能够在全基因组范围内检测转录本的表达情况,为更好的了解基因功能提供线索。本文概述了测序技术的发展,阐述了RNA-seq的相关应用,可为相关研究和实验设计提供参考。
Until today, sequencing technology has achieved a landmark breakthrough. This technology has profoundly altered our understanding of biology diversity, physiological metabolism and disease, meanwhile it has promoted the development of biology. Second-generation sequencing is a commonly used sequence technology which can create a lot of new applications through combining with various experimental methods. RNA-seq is the primary application of second-generation sequencing. It can not only complete genome annotation of genes, but also discover variable splice sites and new genes. Besides, it can also detect transcript expression in the whole genome thus providing clues for better understanding of gene function. This paper systematically summarizes the development of sequence technology and the applications of RNA-seq, which can serve as a reference for related studies and experimental design.
作者
高志勇
谢恒星
李吉锋
刘史力
GAO Zhiyong1,2, XIE Hengxing1,2, LI Jifeng1,2, LIU Shili1(1School of Chemistry and Environment, Weinan Normal University, Weinan 714099, China; 2Key Laboratory for Ecology and Environment of River Wetlands in Shaanxi Province, Weinan 714099, Chin)
出处
《生命的化学》
CAS
CSCD
2018年第1期71-77,共7页
Chemistry of Life
基金
陕西省教育厅2016年重点科学研究计划项目(16JS031)
渭南师范学院理工类人才基金项目(2015ZRRC02)
关键词
测序技术
RNA-SEQ
高通量测序
单分子测序
sequencing technology
RNA-seq
high throughput sequencing
single molecule sequencing
