摘要
为有效利用牛樟芝资源,以人工固体培养牛樟芝菌丝体为原料,采用响应面法优化牛樟芝总三萜超声辅助提取工艺,比色法评价牛樟芝总三萜体外抗氧化活性,滤纸片法研究牛樟芝总三萜的体外抑菌活性。结果表明,超声辅助提取牛樟芝总三萜的最佳工艺条件为:提取时间41 min、提取功率80 W、料液比1∶20,总三萜得率为3.84%±0.18%。牛樟芝三萜提取物具有较强的还原能力,并能有效清除DPPH自由基、ABTS自由基和超氧阴离子自由基,其半清除浓度(EC_(50))分别为0.29、0.50、0.33mg·m L^(-1),且清除率与质量浓度存在剂量效应关系;牛樟芝总三萜对大肠杆菌、金黄色葡萄球菌及枯草芽孢杆菌均具有明显的抑制作用,同浓度下对金黄色葡萄球菌具有较高的抑菌活性。本研究结果为人工固体栽培牛樟芝活性成分的综合开发利用提供了依据。
In order to effectively utilize the resource of Antrodia camphorata, the extraction conditions of total triterpenoids from mycelium of solid-state cultured Antrodia camphorata were optimized by response surface analysis（ RSE）. In vitro antioxidant activities of triterpenoids were evaluated by colorimetry. Antibacterial activities of triterpenoids were determined by filtering paper method. The results showed that the optimum ultrasonic-assisted extraction conditions were as follows： extraction time of 41 min,extraction power of 80 W,and a ratio of solid to liquid as 1： 20. And the yield of total triterpenoids from Antrodia camphorata was 3. 84% ± 0. 18%. The iron ions reduction results showed that triterpenoids had certain reducing power. The EC_（50） values of free radical scavenging capacities of DPPH,ABTS and superoxide anions was 0. 29,0. 50,and 0. 33 mg·m L^-1,respectively. The triterpenoids have strong inhibition effects against Escherichia coli,Staphylococcus aureus and Bacillus subtilis. Higher antibacterial activity was found to be against of Staphylococcus aureus when used at the same concentration of triterpenoids. In conclusion,total triterpenoids extracts from Antrodia camphorata showed relatively strong antioxidant activities and antibacterial activities.This work could provide useful information for further development and comprehensive utilization of total triterpenoids from solid-state cultured Antrodia camphorata.
出处
《核农学报》
CAS
CSCD
北大核心
2018年第1期112-122,共11页
Journal of Nuclear Agricultural Sciences
基金
国家科技支撑计划课题(2013BAD16B00)
浙江省自然科学基金资助项目(LY13C200011)
关键词
牛樟芝
总三萜
超声辅助提取
抗氧化
抑菌
Antrodia camphorata, total triterpenoids, ultrasonic assisted extraction, antioxidant, antibacterial
