摘要
应用单因素试验、析因试验和响应面分析法等统计学方法,对自行设计的可以同时控制温度的微波装置辅助提取美味猕猴桃根中具有保肝作用的三萜类化合物的工艺进行了深入研究。试验结果表明:在微波功率一定的情况下,乙醇浓度、提取温度和提取时间的影响最显著,优化得到最佳微波辅助提取工艺参数为乙醇浓度63%,温度47℃,时间为30 m in,一次提取率可达85.13%。经验证试验证明,该方法是一种实用的提取方法。
Single-factor experiment, fraction factorial design experiment and response surface methodology(RSM) were applied to optimize the extraction conditions of hepatoprotective total triterpenoid from Actinidia deliciosa(A. Chev. ) C. F. Ling et A. R. Ferguson root by using a self-designed temperature-controlled microwave equipment. Results suggest that ethanol concentration, extraction temperature and extraction time are statistically significant factors when microwave power is constant. The optimized conditions are ethanol concentration of 63%, extraction temperature of 47℃ and extraction time of 30 min when microwave power is 450 W. Under the optimized operating conditions, the extraction rate of total triterpenoid is 85.13% with one-time extraction. The predicted value was also verified by validation experiments. The results of this study provided useful information and reference for the optimization of extraction conditions and processes of triterpenoid by microwave-assisted equipment.
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2006年第8期188-193,共6页
Transactions of the Chinese Society of Agricultural Engineering
关键词
美味猕猴桃根
三萜
微波
提取
响应面分析
Actinidia deliciosa (A. Chev. ) C. F. Ling et A. R. Ferguson root
triterpenoid
microwave
extraction
response surface analysis