摘要
目的:研究针刺治疗对抑郁模型大鼠海马谷氨酸脱羧酶65(GAD65)、谷氨酸脱羧酶67(GAD67)的蛋白表达水平,探讨针刺疗法的抗抑郁作用及其相关作用机制。方法:将100只大鼠随机分成5组,空白对照组、氟西汀组、针刺非穴组、针刺穴位组以及模型组,各组均20只,采用慢性综合应激法建立抑郁模型,各组进行相应干预21 d,观察治疗前后大鼠行为学变化。并在实验结束后,从以上5组大鼠中,每组随机抽取8只,Western Blot法检测海马区GAD65、GAD67蛋白表达水平。结果:与空白组相比,实验21 d后,模型组、针刺组、非穴位针刺组的垂直运动次数明显减少,差异有非常显著性(P<0.01),模型组GAD65蛋白表达量降低,差异有显著性(P<0.05);与模型组相比,氟西汀组和针刺组体质量增幅较大、糖水摄入量明显增加、水平运动次数明显增加、垂直运动次数明显增加,差异有非常显著性(P<0.01),针刺穴位组GAD65蛋白表达量升高,差异有显著性(P<0.05);5组间GAD67蛋白表达水平差异无显著性(P>0.05)。结论:对慢性复合应激大鼠百会、三阴交以及内关等穴实施针刺,能够在一定程度上缓解其抑郁状态;穴位针刺的抗抑郁作用可能与增加GAD65合成、减少海马谷氨酸(Glu)含量、增加γ-氨基丁酸(GABA)含量有关。
Objective： to study the effect of acupuncture on regulation of GAD65 and GAD67 expressions in hippocampus of depression rats,and to explore the antidepressant effect and related mechanism of acupuncture therapy. Methods： 100 rats were randomly divided into 5 groups： control group,Fluoxetine group and non-acupoint acupuncture group,acupuncture group and model group,each group has 20 rats. Chronic comprehensive stress method was employed to establish depression models. Each group was given corresponding intervention for 21 d to observe the ethological changes before and after treatment in rats. After the experiment,8 rats in each group were randomly selected from the 5 groups,and the expression of GAD65 and GAD67 protein in the hippocampus was detected by Western Blot. Results： Compared with the control group,after 21 d,vertical motions of model group,acupuncture group and non-acupoint group were significantly reduced,the difference was significant（ P〈0. 01）,and the expression of GAD65 in the model group decreased significantly（ P〈0. 05）; compared with the model group,the body weights of Fluoxetine group and acupuncture group increased considerably,sucrose intake increased significantly,the numbers of horizontal movements and vertical movements increased significantly,and there was significant difference（ P〈0. 01）; the expression of GAD65 of the acupuncture group increased significantly（ P〈0. 05） with great difference; there was no significant difference between GAD67 expressions of the 5 groups（ P〈0. 05）. Conclusion： The implementation of acupuncture on chronic stress rats at DU 20（ bai hui） and SP 6（san yin jiao） and PC 6（ nei guan） can relieve the depression status in a certain extent; the antidepressant effects of acupuncture may be related to increasing the synthesis of GAD65,reducing glutamate（Glu） content,and increasing gamma aminobutyric acid（GABA） content.
出处
《河北中医药学报》
2018年第1期5-8,共4页
Journal of Hebei Traditional Chinese Medicine and Pharmacology
基金
国家自然科学基金项目:No.81260555
云南省科技厅应用基础研究面上项目:No.2011FZ161
关键词
针刺疗法
抑郁症
谷氨酸脱羧酶65
抗抑郁
氟西汀胶囊
acupuncture
depression
glutamic acid decarboxylase （ GAD） 65
anti-depression
Fluoxetine capsule
