摘要
本试验旨在探究氧化应激状态下猴头菇多糖(HEP)对断奶仔猪回肠形态结构及通透性的影响。将45头健康的(28±3)d断奶仔猪按体重相近原则分为5组,空白组和模型组饲喂基础日粮,试验组在基础日粮中分别添加0.2%、0.4%、0.6%的HEP。于试验第21天,对模型组和HEP组按体重注射100μg·kg-1脂多糖(LPS),空白组注射等剂量灭菌生理盐水;前腔静脉采血15mL,分离血清,用于检测抗氧化指标和肠黏膜完整性指标;宰杀试验猪,分离回肠,HE染色观察其形态变化;qPCR和Western blot法检测回肠紧密连接蛋白的表达情况。结果显示,同模型组比较,HEP能显著改善LPS应激造成的血清抗氧化指标下降(P<0.05);显著提高仔猪血清中谷氨酰胺(GLN)含量(P<0.05),降低二胺氧化酶(DAO)活性(P<0.05),缓解LPS导致的仔猪回肠黏膜损伤;使得LPS应激诱导的回肠上皮细胞紧密连接蛋白表达下降显著回升(P<0.05)。饲料中添加HEP能够有效改善氧化应激状态下仔猪的回肠形态结构及通透性。
This study aims to investigate the effects of feeding Hericium erinaceus polysaccharide （HEP） to weaned piglets on ileal morphology and permeability under oxidative stress. Forty-five healthy long-white weaned piglets with similar weight were randomly divided into five groups （every group had 9 replications）. Control group and model group were fed with basic diet only, meanwhile the other three test groups were fed with basic diet added with 0.2%, 0.4% or 0.6% HEP. On the 21st day, piglets in model group and three test groups were injected with 100 μg·kg-1·BW lipopolysaccharide, meanwhile, piglets in control group were injected with equal dose sterilized normal saline. Blood samples were collected to detect antioxidant functions and intestinal mucosal integrity. The piglets were slaughtered and the ileums were collected to observe the morphological changes by HE staining; the expression of tight junction proteins of ileum were detected by qPCR methods and Western blot methods. The results showed that, compared with the model group, HEP could significantly improve the decrease of serum antioxidant indexes caused by LPS （P〈0.05）, significantly increase glutamine （GLN） content （P〈0.05） and reduce diamine oxidase （DAO） activity （P〈0.05）; and significantly recovery the decrease of tight junction protein expression in ileum epithelial cells induced by LPS stress （P〈0.05）. Supplementation of HEP can effectively improve the permeability of ileal morphology under oxidative stress.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2018年第1期203-210,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(31502110)
关键词
猴头菇多糖
断奶仔猪
氧化应激
回肠形态结构
紧密连接
Hericium erinaceus polysaccharide
weaned piglets
oxidative stress
ileal morphology
tight junction
