摘要
目的比较乳杆菌、双歧杆菌、嗜热链球菌和乳酸乳球菌的抗氧化活性,分析抗氧化活性较高菌株内与抗氧化相关的成分。方法采用清除DPPH自由基、清除羟自由基以及抗脂质过氧化能力3种方法评价40株益生菌的抗氧化活性,选出抗氧化活性较高的菌株分析其超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和总巯基化合物(TTG)的含量。结果所比较的40株益生菌的抗氧化能力差别较大,既具有种属特异性,又具有菌株特异性;7株嗜热链球菌和11株乳酸乳球菌中的SOD活性最高[(平均分别为124.02U/mg(蛋白质)和107.10U/mg(蛋白质)]、2株双歧杆菌中GSH的含量最高(平均为311.03μmol/L细胞破碎液),所分析的菌株中TTG含量均较高。研究结果表明,实验菌乳酸乳球菌清除自由基能力最强,其次是嗜热链球菌,所试验的乳杆菌和双歧杆菌最弱;SOD对益生菌的抗氧化起主要作用;GSH的存在因种属和菌株的不同而差别较大;TTG对益生菌的抗氧化起了积极作用。
To examine tococcus lactis and analyze ods Antioxidative activity radicals and anti-lipid per the antioxidation of lactobacilli, bifidobacteria, Streptococcus thermophilus, Lacinternal correlative antioxidative components of high antioxidative strains. Methods Antioxidative activity of probiotics was evaluated by scavenging DPPH free radicals, scavenging hydroxyl oxidation. The content of SOD, GSH and TTG （total thiol groups） for high antioxidative strains was then determined. The results show that the antioxidation of 40 probiotics strains are different, they are genus-specific and strain-specific; seven Streptococcus thermophilus strains and eleven Lactococcus lactis strains have the highest SOD activity （124.02 U/mg protein, 107.10 U/mg protein）, two bifidobacterial strains have the most content of GSH （311.03μmol/L cell-free extracts of probiotics）, the content of TTG is high for all the tested strains. The level of scavenging free radicals is the highest for Lactococcus lactis, secondary for Streptococcus thermophilus, and low for lactobacillus and bifidobacteria; SOD has mainly contributed to the antioxidation of probiotics; the existing GSH is largely different in its genusspecific and strain-specific; TTG has active effect on the antioxidation of probiotics.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2009年第5期6-11,共6页
Food and Fermentation Industries
基金
国家863项目资助(2008AA10Z335)
