摘要
本试验选用28日龄杜长大断奶仔猪135头,按随机区组设计分成5组;对照组饲喂基础日粮,试验Ⅰ、Ⅱ、Ⅲ组分别在基础日粮中添加0.5%、1.0%、2.0%的中草药提取物,抗生素组在基础日粮中添加20mg/kg硫酸粘杆菌素和100mg/kg杆菌肽锌,研究中草药复方对早期断奶仔猪生长性能和肠道菌群的影响。结果表明:中草药组的腹泻率比对照组分别降低47.6%、51.2%和45.2%(P<0.05),与抗生素组无显著差异(P>0.05)。肠道菌群DGGE图谱显示,中草药组仔猪直肠粪样相似性指数大于60%,0.5%中草药组微生物多样性最高;DGGE图谱中3条特异条带的序列与GenBank登录的相关序列的相似性都大于90%。
To study the effects of Chinese herbs as feed additives on growth performance and intestinal bacterial diversity on piglets.135 weaned piglets（Duroc × Landrace × Yorkshire） were used in a 28-day growth trial.The piglets were allocated to five dietary treatments in a randomized complete block design.The dietary treatments were control group（basal diets）;treatments of Ⅰ,Ⅱ,Ⅲ,adding 0.5%,1.0% and 2.0% of herb extract into the basal diets;antibiotic group,adding 20 mg/kg Colistin Sulfate and 100 mg/kg Bacitracin Zinc into the basal diet.The results showed that：comparing to the control group,supplementation with herb additives decreased the diarrhea by 47.6%,51.2% and 45.2%（P〈0.05）,and no significant difference in the antibiotic group（P〉0.05）.PCR-DGGE DNA profiles of the V6-V8 region gene of 16S rDNA of microbiota in rectum indicate the degree of similarities of Chinese herb groups were exceeding 60%,the high levels of diversity were found in 0.5% herb group;These bands indicating with a-c observed in herb groups showed exceeding 90% similarity with known sequences of Genbank.
出处
《中国畜牧杂志》
CAS
北大核心
2010年第3期31-35,52,共6页
Chinese Journal of Animal Science
基金
杭州市科技创新基金项目(20051322B34)
关键词
中草药添加剂
生长性能
腹泻
肠道微生物
断奶仔猪
Chinese herb additives
growth performance
diarrhea
intestinal microflora
weaned piglet
