摘要
miR408家族在植物上已有报道,但其进化规律与分子特性研究很少.利用miRbase数据库提供的34种植物49个miR408的前体序列和64个成熟体序列,采用分类统计、进化树构建、序列比对、二级结构预测、染色体定位分析及靶基因预测等手段,进行进化规律与分子特性分析.结果显示:miR408家族成员分布在34个植物物种中,分布较为广泛,并且苔藓类植物可能是植物miR408家族进化的祖先;物种特异性是影响miR408前体进化特性的重要因素,而序列保守性是影响成熟体进化特性的主要因素;由5p臂上形成的miR408成熟体的序列特异性较大,由3p臂上形成的miR408成熟体的序列保守较高;Mfold预测表明植物miR408家族具有典型的茎环二级结构,茎序列的保守性高于环序列,3p臂上茎序列的保守性高于5p臂上茎序列;靶基因预测表明miR408广泛参与植物生长发育和逆境胁迫的应答.综上表明植物miR408家族成员在进化过程中保守性与特异性并存,这对进一步研究植物miR408的生物学功能具有参考价值.
The miR408 family has been reported in some plant species, but little is known about its evolutionary rules and molecular characteristics. In this study, based on the miRbase, 49 precursors and 64 mature sequences of the miR408 family in 34 plant species were analyzed using distribution statistics, construction of phylogenetic trees, sequence alignment, prediction of the secondary structures, analyses of chromosome location, and prediction of the target genes, to explore the molecular characteristics and evolution of the miR408 family members. The results show that the miR408 family members were widely distributed in all 34 species of plants, which suggests that bryophytes might be the evolutionary ancestors of the miR408 family. The specificity of species was an important factor affecting the precursor evolution of the miR408 family, whereas the conservation of sequences was one of the main factors affecting the evolution of the mature miR408 family. The members of miR408-5p were quite specific, whereas the members of miR408-3p were conserved. Mfold predicted that pre-miR408 spontaneously forms a typical, stable stem-loop secondary structure and the stem sequence was more conserved than the loop sequence, and the miR408-3p sequence was more conserved than the miR408-5p sequence. Target gene analysis showed that miR408 family members played various roles in regulating plant growth, development, and response to stress in plants. The miR408 family members were coexistent with conservatism and specificity in evolution, which is a valuable reference？for exploring the diverse biological functions of the miR408 family in plants.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2017年第6期1042-1051,共10页
Chinese Journal of Applied and Environmental Biology
基金
福建省重大科技专项(2015NZ0002-1)
国家现代农业产业产业技术体系(香蕉)专项资金(CARS-31-15)资助~~
关键词
植物
miR408家族
进化规律
分子特征
靶基因预测
plant
miR408 family
evolutionary regularity
molecular characteristic
target gene predict
