摘要
为了探讨局灶性脑缺血再灌注大鼠采用Ephrin-B2干预促进大鼠神经功能的恢复及作用机制,本研究选取了72只成年雄性SD大鼠,采用随机数字表法分为:空白组(等量生理盐水)、假手术组(等量生理盐水)、模型组(等量生理盐水)和干预组(Ephrin-B2连续干预3 d),每组各18只。通过对比各组大鼠神经功能评分,检测对比各组大鼠缺血侧大脑海马组织中血管细胞间黏附分子-1(VCAM-1)、核转录因子(NF-κB)蛋白、微血管密度的水平,检测并比较各组大鼠缺血侧海马组织中血管内皮生长因子(VEGF)m RNA及蛋白的表达水平,我们发现造模后第4、8、12、14天,干预组大鼠的神经功能评分显著的低于模型组(p<0.05);造模后第4、8、12、14天,干预组、模型组大鼠的缺血侧大脑海马组织中VCAM-1、NF-κB蛋白显著的高于空白组和假手术组(p<0.05);干预组大鼠的缺血侧大脑海马组织中VCAM-1、NF-κB蛋白显著的低于模型组(p<0.05);造模后第4、8、12、14天,干预组大鼠的缺血脑组织中微血管密度显著的高于模型组(p<0.05);造模后第4、8、12、14天,干预组、模型组大鼠的血侧脑组织VEGF m RNA及蛋白显著的高于空白组和假手术组(p<0.05);干预组大鼠的血侧脑组织VEGF m RNA及蛋白显著的高于模型组(p<0.05)。本研究表明,局灶性脑缺血再灌注大鼠采用Ephrin-B2干预能促进大鼠神经功能的恢复,可能与促进新生血管形成有关。
In order to investigate the effect of Ephrin-B2 intervention on the recovery of neurological function in rats with focal cerebral ischemia and reperfusion, we selected 72 adult male SD rats were randomly divided into control group（saline）, sham operation group（saline）, model group（saline） and intervention group（intervention Ephrin-B2 for continuous 3 days）, with 18 rats in each group. With the comparison of neural function score in rats,vascular cell adhesion molecule-1（VCAM-1）, a nuclear transcription factor（NF-κB） protein level, and microvessel density were detected and compared in hippocampus of brain tissue bleeding side, vascular endothelial growth factor（VEGF） m RNA and protein expression level were detected in the hippocampus tissue of ischemia. We found that four, eight, twelve, and fourteen days after modeling, neural function score in the intervention group was significantly lower than that in the model group（p〈0.05）. Four, eight, twelve, fourteen days after modeling, VCAM-1,NF-κB protein in hippoca mp us of brain hemorrh age side in the model group and intervention group weresignificantly higher than those of blank group and sham operation group（p〈0.05）. VCAM-1, NF-κB protein of brain hemorrhage in the intervention group were significantly lower than those in the model group（p〈0.05）. Four, eight,twelve, 14 days after modeling, microvessel density of ICH in the intervention group was significantly higher than that in model group（p〈0.05）. Four, eight, twelve, 14 days after modeling blood, VEGF m RNA and protein in intervention group and model group were significantly higher than those in the control group and sham operation group（p〈0.05）. In addition, VEGF m RNA and protein of blood brain tissue were significantly higher than in the intervention group compared with those in model group（p〈0.05）. Our research indicated that the intervention of Ephrin-B2 could promote the recovery of neurological function in rats with focal cerebral ischemia and reperfusion, which might be associated with the form of neovascularization.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第12期4906-4913,共8页
Genomics and Applied Biology
基金
哈励逊国际和平医院资助
