摘要
将家兔染色体DNA用限制性内切腐Hind Ⅲ晦切得到的高重复顺序DNA最小片段重组到质粒pUC12上,转化受体菌JM101后经过筛选和杂交鉴定,得到含家兔高重复顺序DNA片段的重组质粒克隆,用末端终止法测得此克隆片段(命名为RAb(Hind Ⅲ)-1)全部核苷酸顺序为345个bp,用计算机对实验数据进行分析比较,结果表明该序列的结构特征与灵长类动物类α顺序安全不同,对此进行了讨论。
We obtained highly repeated DNA fragments from a rabbit by using restric- tion endonuclease HindⅢ digestion of chromosomal DNA; the smallest highly repeated DNA fragment was cloned into vector plasmid pUC12. We used the recombinant plasmid to transform E. coli JM101, selected and identified the positive clone by DNA probe in situ hybridization as well as Southern blot- ting. We obtained a recombinant clone [termed pRAb (Hind Ⅲ)--1] which con- tains the highly repeated DNA fragment [termed RAb (Hind Ⅲ)--1]. The com- plete nucleotide sequence of the 345bP RAb(Hind Ⅲ)--1 fragment was determined by the dideoxynucleotide termination sequencing method. We analyzed the characteristics of the structure of the sequence with a microcomputer, and the results suggested that the RAb (Hind Ⅲ)--1 sequence is quite different from alphoid DNA of primates.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1991年第4期241-245,共5页
Acta Academiae Medicinae Sinicae
关键词
DNA
分子克隆
核苷酸
highly repeated DNA of rabbit α-DNA alphoid DNA α-type DNA
