摘要
目的 观察知母皂苷元 (ZMS)对拟痴呆大鼠模型脑内 β 淀粉样肽沉积及胆碱能系统功能的作用。 方法 单侧基底核内联合注射 β 淀粉样肽2 5-3 5片段 (β AP2 5-3 5)和兴奋性氨基酸建立大鼠拟痴呆模型 ,然后将模型动物分为假手术组 37、模型组和ZMS组 ,采用免疫组织化学方法和图像分析法测定β AP2 5-3 5沉积斑块的面积。用避暗法及跳台法测定动物的学习记忆功能 ,用放射配基结合分析法分别测定脑胆碱乙酰转移酶 (ChAT)活性和M受体密度。结果 一次性脑内联合注射 β AP +IBO后 ,模型大鼠脑内有明显的 β AP斑块沉积 ,而模型大鼠喂服ZMS 6 0d后 ,能有效地减少β AP沉积的面积 ,同时ZMS能明显改善模型动物的学习记忆功能 ,并提高模型动物脑内ChAT活性和M受体密度。结论 ZMS可能对脑内 β AP的沉积有一定的清除作用 。
AIM To observe the effects of ZMS active component of Zhimu on deposition of β amyloid peptide and cholinergic function in rats with β amyloid injection into right nucleus basalis magnocellularis (NBM). METHODS A combined injection of β AP 25-35 and excitatory amino acid into the right NBM was used as a dementid like rat model and model animals were divided into sham operation group, model group and ZMS group. The area of depositive spots of β AP 25-35 was detected by the ABC immunohistochemical and the image analysis methods. The learning and memory abilities were detected by step down test and step through test, the activity of brain choline acetyltransferase (ChAT) and the muscarinic receptor (MR) density were determined by 3H acetyl CoA binding analysis and 3H QNB binding tests respectively. RESULTS It was found that the deposition of β AP 25-35 in nucleus basalis increased, 30 d to 60 d after combined injection; while the cleposition decreased in model rats with once a day oral administration of ZMS for 60 d. ZMS significantly improved the learning and memory ability of the model animals. It also increased the brain activity of ChAT and the brain muscarinic receptor density of these animals. CONCLUSION These results suggeste that ZMS may help in scavenging action for the deposition of β AP 25-35 and in improving and treating the cholinergic disfunction in Alzheimers disease.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2002年第4期390-393,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目
No 3 95 70 869
关键词
知母皂苷元
痴呆
大鼠
Β-淀粉样肽
沉积
胆碱能系统功能
sapogenin from Zhimu(ZMS)
β amyloid peptide
dementia like rat model
immunohistochemistry
ChAT
