摘要
目的 探讨恶性淋巴瘤mdr1、MRPmRNA和P gp表达水平与化疗疗效的相关性。方法 应用半定量逆转录多聚酶链反应 (RT PCR)技术和流式细胞术 (FCM)方法 ,以 8例人正常淋巴结为正常对照 ,对 4 6例淋巴瘤患者 [2 3例初治 (HD1例 ,NHL2 2例 )及 2 3例复发 (HD5例 ,NHL18例 ) ]的mdr1mRNA、MRPmRNA和P gp表达水平与化疗疗效间的关系进行了前瞻性研究。结果 复发患者mdr1基因和P gp表达水平及阳性率均高于初治患者 (P <0 0 0 1) ,而MRP基因表达水平及阳性率在复发与初治患者间差异无显著意义 (P >0 0 5 )。mdr1基因及P gp表达阳性患者的化疗有效 (CR +PR)率 (33 33%和 2 6 6 7% )明显低于mdr1基因及P gp表达阴性患者 (85 71%和 83 87% ,P <0 0 0 1) ,而MRP基因表达阳性患者与阴性患者的化疗有效率差异无显著意义 (P >0 0 5 )。相关分析显示 ,mdr1基因和P gp表达水平之间有明显相关性 (r=0 2 96 3,P <0 0 5 ) ,而mdr1和MRP之间、MRP与P gp之间均无明显相关性 (r =0 0 72 3,P >0 0 5 ;r=0 0 818,P >0 0 5 )。结论 mdr1基因及P gp表达是恶性淋巴瘤患者多药耐药的主要机制 ,而MRP基因不是产生耐药的主要机制。mdr1基因及P gp表达水平的高低与恶性淋巴瘤化疗疗效密切相关 。
Objective To explore the correlation of chemotherapy efficacy in malignant lymphomas with the expression level of multidrug resistance gene 1 (mdr1) mRNA, multidrug resistance associated protein (MRP), and P glycoprotein (P gp). Methods Using the methods of sem iquantitative reverse transcriptase polymerase chain reaction (RT PCR) and flow cytometry (FCM), a prospective research was performed to detect the expression levels of molrl gene,MRP gene,and p gp in the lymph nodes of 23 untreated first visit (HD1,NHL 22)and 23 recurrent (HD 5, NHL 18) lymphoma patients. Eight normal human lymph nooles were used as controls. Results The expression levels and positive rates of mdr1 and P gp in recurrent patients were higher than those in untreated patients ( P <0.001). There was no difference in MRP gene expression level and positive rate between recurrent and untreated patients ( P >0.05). The chemotherapy effective rates were lower in mdr1 gene and P gp positive expression patients (33.33% and 26.67%) than in negative expression patients (85.71% and 83.87%) ( P <0.001). Relevant analysis showed that there was a significant relevance in expression level between mdr1 gene and P gp ( r =0.296 3, P <0.05), but there was no correlation between mdr1 and MRP or between MRP and P gp (r=0.072 3, P >0.05; r =0.081 8, P >0.05). Conclusion mdr1 gene and P gp expression levels are dominant mechanisms of clinical drug resistance of lymphomas, whereas, MRP gene appears to play no role in that course. mdr1 gene and P gp expression levels are relevant to chemotherapy efficacy, however, MRP gene expression level seems to have no impact on it.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2002年第17期1177-1179,共3页
National Medical Journal of China
基金
山东省科委研究基金资助项目 (9712 2 62 0 7)
