摘要
目的:建立高效液相色谱法检测产妇母乳和血浆中氟比洛芬的方法,用于评估产妇乳汁/血药浓度百分比。方法:以卡马西平为内标,乳汁和血浆样品加入1 mol·L^(-1)的盐酸溶液后用正己烷∶乙酸乙酯(1∶4)萃取,选择C_(18)柱检测,以甲醇-6.85 mmol·L^(-1)磷酸-乙腈(30∶40∶30)为流动相,流速为1.0 mL·min-1,检测波长为247 nm,柱温为30℃。结果:乳汁和血浆中氟比洛芬的浓度分别在0.02~5.00μg·mL^(-1)(R^2=0.999 7)和0.05~10.00μg·mL^(-1)(R2=0.999 2)内线性关系良好,日内、日间RSD均低于6%。20例产妇24,36,48 h的乳汁/血药浓度百分比分别为6.87%,6.33%,6.58%。结论:该方法快速、简便、灵敏度高、重现性好。氟比洛芬在乳汁中分布较少。
OBJECTIVE To establish a HPLC method for the determination of flurbiprofen in maternal breast milk and plas- ma, and assess the milk-to-plasma ratio in pregnant women. METHODS With carbamazepine as internal standard, milk and plasma samples were added to 1 mol.L-1 hydrochloric acid solution and were extracted with hexane： ethyl acetate （1：4）. The analysis was conducted on C18 column, with the mobile phase of methanol - 6. 85 mmol·L^-1 phosphoric acid- acetonitrile （30：40：30）, the flow rate at 1.0 mL. min 1 the detection wavelength at 247 nm, and the column temperature at 30%. RESULTS Milk and plasma concentrations of flurbiprofen were 0.02 - 5.00 μg.mL-1 （R2 = 0. 999 7） and 0.05 - 10. 00μg. mL-1 （R2 = 0. 999 2）, in good linear relationship. The intra- and inter-day precisions were both less than 6%. The milk-to-plas- ma ratios were 6. 87%, 6. 63% and 6. 58% in 20 pregnant women at 24, 36, 48 h.CONCLUSION The method is rapid, sim- ple, has high sensitivity and good reproducibility. The breast milk only contains a little flurbiprofen.
出处
《中国医院药学杂志》
CAS
北大核心
2017年第22期2255-2258,共4页
Chinese Journal of Hospital Pharmacy
基金
温州市医药卫生科学研究项目(编号:2015B53)
关键词
氟比洛芬
高效液相色谱法
血药浓度
乳药浓度
产妇
flurbiprofen
HPLC
blood concentration
concentration in breast milk
pregnant woman
