摘要
目的建立剖宫产产妇乳汁中枸橼酸芬太尼浓度测定的HPLC方法,并考察剖宫产产妇应用枸橼酸芬太尼镇痛泵镇痛后,芬太尼在乳汁中的分布情况,探讨剖宫产术后新生儿的合理哺乳时间。方法1mL乳汁中加入0.5mol·L-1NaOH200μL碱化,涡旋30s,再加入环己烷5mL,涡旋2min,于2000r·min-1离心20min,静置10min,取上述有机相4mL于另一干净试管中,在N2流下吹干,残渣用流动相350μL溶解,进样量100μL。采用AgillentZorbaxSB-C18柱(4.6mm×250mm,5μm),以0.015mol·L-1磷酸二氢钠-乙腈(60∶40)为流动相,流速为0.7mL·min-1,于200nm波长下检测。结果枸橼酸芬太尼在40～2000μg·L-1内线性关系良好(r=0.9997),方法回收率为85.3%～101.6%,乳汁低、中、高3种浓度的萃取回收率(n=5)分别为62.7%,66.3%,65.6%;RSD(n=5)分别为8.11%,6.72%,4.83%;方法回收率(n=5)分别为86.1%,96.4%,103.7%,日内和日间精密度RSD小于12%。结论本法操作简单,灵敏,准确,重现性好,能满足临床对芬太尼乳药浓度的测定,可用于芬太尼在乳汁中的浓度分布研究。
OBJECTIVE To develop a simple HPLC method for the determination of fentanyl in the breast milk of caesarean women after patient-control analgesia. METHODS 200 μL of NaOH （0.5 mol·L^-1 ） was addetl into 1 mL human milk. After the sample was extracted by 5 mL of cyelohexane, 4 mL organic layer was transferred to a clean tube and evaporated to dryness under a continues nitrogen stream in a waterbath at 40 ℃. The residuel was reconstituted in 350μL of mobile phase and 100 μL was injected into an Agillent SB-C18 HPLC column （4.6 mm× 250 mm, 5 μm）. The mobile phase consisted of 0.015 mol·L^-1 NaH2PO4-acetonitrile （60： 40）at a flow rate of 0.7 mL·min^-1 .The detection wavelength was 200 nm. RESULTS The linear range was 40-2 000μg·L^-1 （r=0.999 7）, and the limit of determination was 125 μg·L^-1 extraction recoveries of low, medium and high concentration were 62.7%, 66.3% and 65.6%. The method recoveries were 86.1%, 96.4% and 103.7%, and the RSDs of Within-day and between-day were below 12%. CONCLUSION This method is simple, sensitive for the determination of fentanyl in the milk of cesarean women after patient-control analgesia, it is applicable for the pharmacokinetic study of fentanyl.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2009年第13期1022-1024,共3页
Chinese Pharmaceutical Journal
基金
浙江省教育厅资助项目(20040276)
关键词
芬太尼
乳汁
fentanyl
women latex
