摘要
目的分析哈尔滨某三甲医院丙型病毒性肝炎患者的HCV基因分型情况,为地区性丙型肝炎的防治提供理论依据。方法收集50例抗-HCV阳性患者血清标本,提取HCV RNA进行核心(core)片段扩增和DNA测序。根据测序结果,采用进化树方法对所得基因序列进行基因分型,获得入组丙型肝炎患者的基因分型情况。结果本研究中中42例患者的血清样本成功扩增出HCV core基因部分片段(+328 nt^+726 nt),其中各种HCV基因分型分布如下:基因分型结果1b型12份(28.57%),2a型29份(69.05%),3a型1份(2.38%)。结论哈尔滨某三甲医院丙型肝炎患者HCV的基因型最常见为HCV 2a型,其次为HCV 1b型,偶见HCV 3a型。
Objective To investigate the HCV genotype of patients with hepatitis C in a tertiary hospital in Harbin, and to provide theoretical basis for the prevention and treatment of regional HCV. Methods Serum samples were collected from 50 cases with anti-HCV positive, and HCV RNA was extracted for core fragment amplification and DNA sequencing. The genotype of the DNA sequence was performed by cladogram and then the genotype of HCV were obtained. Results The core fragment （＋328 nt-＋726 nt） of HCV was amplified in 42 cases among the 50 patients. There were 12 patients had genotype 1b （28.57%）, 29 patients had genotype 2a （69.05%）, 1 patient was 3a （2.38%）. Conclusion The genotype of HCV 2a was the most common type in HCV patients in this hospital, followed by HCV 1b and occasionally HCV 3a.
作者
黄超群
王福祥
Huang Chaoqun;Wang Fuxiang(Department of Gastroenterology, the 211 Hospital of PLA, Harbin 150086, China;Department of Infectious Diseases, The 4th Affiliated Hospital of Harbin Medical University, Harbin 150001, China)
出处
《中华实验和临床感染病杂志（电子版）》
CAS
2017年第5期483-489,共7页
Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)
