摘要
目的:探讨重庆地区丙型肝炎病毒(HCV)流行的基因亚型分布状态.方法:在77份抗HCV抗体和HCVRNA均阳性的血清标本中,分别提取HCVRNA,通过逆转录巢式PCR(RTnestedPCR)扩增C基因的羧基端至E1基因的氨基端长度为474bp的片段,测定其核苷酸序列,与GenBank中已知的HCV序列进行系谱分析,确定HCV基因亚型.结果:77份HCV感染者血清标本通过RTnestedPCR检测均获得阳性条带,该方法特异性和敏感性均较好.序列与系谱分析显示1b29例(38%),2a10例(13%),3a12例(16%),3b11例(14%),6a15例(19%),未见1a和2b型.结论:重庆地区HCV流行的基因亚型有1b,2a,3a,3b,6a共5种,1b为流行的主要基因亚型.2a,3a,3b和6a型均占相当比例,初步说明重庆地区HCV流行的基因亚型呈现多样性.
AIM: To investigate the distribution status of hepatitis C virus subtypes in Chongqing region. METHODS: Serum specimens in this study were obtained from 77 patients whose anti-HCV and HCV RNA were positive. A reverse transcriptase PCR (RT- PCR) assay using conserved primers deduced from the core-envelope 1 (C-E1) region of the hepatitis C virus (HCV) genome was employed to amplify a 474- nucleotide-long fragment. Phylogenetic analysis of the C-E1 sequences was conducted by direct sequencing of the RT-PCR products and alignment with published HCV subtypes in GenBank. Subtypes of all the samples were determined by nucleotide sequencing followed by composition of a phylogenetic tree. RESULTS: All the electrophoretic fragments of HCV genotyping were positive. It was demonstrated that there were five different subtypes of HCV in Chongqing region, i.e. 1b, 29 cases (38%), 2a, 10 cases (13%), 3a, 12 cases (16%), 3b, 11 cases (14%), and 6a, 15 cases (19%). Two previously reported subtypes, 1a and 2b, were not found. CONCLUSION: The HCV subtypes found in Chongqing region are 1b, 2a, 3a, 3b and 6a. HCV subtype 1b, the main subtype, and subtypes 2a, 3a, 3b and 6a are of some proportions.
出处
《第四军医大学学报》
北大核心
2005年第14期1253-1256,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金(30371663)
关键词
肝炎病毒
基因型
亚型
系谱分析
重庆
hepacivirus
genotype
subtype
phylogenetic analysis
Chongqing
