摘要
[目的]研究银杏叶叶绿素的荧光特征及其含量的变化规律。[方法]以新鲜银杏叶为材料,采用混合有机溶剂进行萃取,并用分光光度法对银杏叶叶绿素的含量进行测定,通过比较不同月份银杏叶叶绿素的含量,得到叶绿素含量的变化规律,同时研究银杏叶叶绿素的特征荧光发射峰和磷光寿命。[结果]银杏叶叶绿素有2个特征荧光发射峰,分别位于691和718 nm处,在2个发射峰处的磷光寿命分别为8.61和8.21μs;在不同月份的银杏叶中,叶绿素含量从大到小依次为7月、4月、11月,但叶绿素a/b比值从大到小依次为4月、11月、7月。[结论]该研究为银杏叶叶绿素的进一步研究提供参考。
[ Objective ] The research aimed to study fluorescence characteristics and content variance of chlorophyll in ginkgo leaves. [ Meth-od ]Fresh ginkgo leaves were used as raw material, chlorophyll was extracted by mixed organic solvent and its content was measured with spec-trophotometry. Chlorophyll contents in ginkgo leaves in different months were compared to obtain variance of chlorophyll content, and charac-teristic fluorescence peaks and phosphorescence lifetimes of chlorophyll were studied. [Result] The chlorophyll of ginkgo leaves had two char-acteristic fluorescence peaks and they were located at 691 nm and 718 nm, and the phosphorescence lifetimes of chlorophyll of ginkgo leaves were 8.61 and 8.21 （ jls at two emission peaks respectively. The chlorophyll content in ginkgo leaves in July was the highest, and that in April ranked the second,and that in November was the lowest. However, the order of chlorophyll a/b value in different months was April 〉 Novem-ber 〉 July. [ Conclusion] This study provides guidance for further research of chlorophyll in ginkgo leaves.
出处
《安徽农业科学》
CAS
2017年第31期134-136,共3页
Journal of Anhui Agricultural Sciences
关键词
银杏叶
叶绿素
荧光特征
含量测定
分光光度法
Ginkgo leaves
Chlorophyll
Fluorescence characteristics
ntent determination
Spectrophotometry
