摘要
目的探讨西格列汀对NOD小鼠胰岛炎的改善作用与其作用机制。方法将22只3月龄雌性NOD小鼠随机分为西格列汀组与对照组(n=11),西格列汀组每日给予磷酸西格列汀灌胃(30 mg/kg),对照组给予等体积生理盐水,连续处理12周,每周测量小鼠体质量、摄食量、饮水量和血糖值,第13周行腹腔糖耐量试验(IPGTT),ELISA检测血清胰岛素水平,HE染色观察胰岛形态及炎症细胞浸润情况,免疫印迹法(Western Blot)检测TLR4、MyD88和NF-κB蛋白表达水平。结果西格列汀组小鼠血清胰岛素水平较对照组高(P<0.05),胰岛炎症细胞浸润状况显著改善(P<0.05),TLR4、MyD88及NF-κB蛋白表达水平显著低于对照组(P<0.05)。结论西格列汀显著减轻NOD鼠胰岛炎症浸润情况、增加血清胰岛素水平,这可能与其抑制TLR4/NF-κB信号通路相关。
Objective To explore the effects of DPP-4 inhibitor sitagliptin on insulitis of NOD mice andthe potential mechanism. Methods 22 3-month-old female NOD mice were randomly divided into Sitagliptin（Si-ta）group（n = 11）gavaged with Sita（30 mg/Kg）daily for 12 weeks and control（Con）group with equal volume ofnormal saline（NS）. The body weight,food-intake,water-intake and blood glucose were recorded weekly. Intraperitoneal glucose tolerance test（IPGTT）was performed at the end of treatment and the blood sample was collected.Then mice were execute. The serum insulin level was measured by ELISA. Pancreas morphology and insulitis were evaluated by hematoxylin-eosin staining. The protein expression level of TLR4,MyD88 and NF-κ B was analyzed by Western Blot. Results Compared with that of Con group,the insulitis of Sita group was significantly alleviative（P 0.05）and the serum insulin level was increased significantly（P 0.05）. The protein expression level of TLR4（P 0.05）,MyD88（P 0.01）and NF-κB（P 0.05）in Sita group was significantly decreased.Conclusion Sitagliptin alleviates insulitis in NOD mice and increases the serum insulin level,probably owing to the suppression of TLR4/MyD88/NF-κB signaling pathway.
出处
《实用医学杂志》
CAS
北大核心
2017年第20期3379-3383,共5页
The Journal of Practical Medicine
基金
广州医科大学青年科研项目(编号:2014A07)
广州市属高校科研项目(编号:1201630638)
