摘要
目的探讨MiR-155调控Smad5在糖尿病肾病(DN)肾脏纤维化的作用。方法应用荧光定量PCR及原位杂交检测miR-155在DN肾组织中的表达。采用生物信息学预测miR-155的靶基因,并通过Western Blot及检测双荧光素酶报告基因活性进行验证。采用Western Blot检测DN系膜细胞增殖及系膜基质相关标志蛋白的表达。结果 (1)MiR-155在DN肾组织及高糖刺激的肾实质细胞中均表达升高。(2)MiR-155靶向调控Smad5基因表达。(3)MiR-155通过下调Smad5表达促进系膜细胞增殖及细胞外基质增加。结论 MiR-155通过调控Smad5基因促进系膜细胞增殖及肾脏纤维化,为深入认识DN的发病机制提供了依据。
Objective To investigate the role of miR-155 in diabetic nephropathy（DN）and its mecha-nism. Methods MiR-155 expression level in kidney was detected by real-time PCR and in situ hybridization. Thetarget gene of mi R-155 was predicted by bioinformatics and verified by Western Blot and double luciferase reporteractivity. Western Blot was used to detect the related marker proteins of mesangial cells proliferation and mesangialmatrix. Results（1）The expression of mi R-155 increased in DN renal tissue and high glucose-stimulated renalcells.（2）MiR-155 was related to the regulation of Smad5 gene expression.（3）MiR-155 promoted the mesangialcells proliferation and increased extracellular matrix by down-regulating Smad5 expression. Conclusions MiR-155 can promote the mesangial cells proliferation and renal fibrosis by regulating Smad5 gene,providing a basis forfurther understanding the pathogenesis of DN.
出处
《实用医学杂志》
CAS
北大核心
2017年第20期3340-3344,共5页
The Journal of Practical Medicine
基金
国家自然科学基金(编号:81600654)
广东省自然科学基金(编号:2014A030310060
2016A030313490)
