摘要
目的:检测食管鳞癌患者外周血中程序性死亡分子1(programmed cell death 1,PD-1)、程序性死亡分子1配体(programmed cell death ligand 1,PD-L1)及IFN-γ表达情况,并分析其临床意义。方法选取2016年6月至2017年4月河北医科大学第四医院胸外科90例食管鳞状细胞癌患者(其中50例患者行手术治疗)和40例健康对照者为研究对象,收集研究其外周血液标本,采用酶联免疫吸附方法检测血清中可溶性PD-1(sPD-1)、可溶性PD-L1(sPD-L1)及IFN-γ的表达水平。采用SPSS 24.0软件对数据进行检验和相关性分析。结果:食管鳞癌组血清中sPD-1、sPD-L1及IFN-γ水平均明显高于正常对照组(P<0.05);食管鳞癌组手术前血清sPD-L1、IFN-γ水平均明显高于术后(P<0.05),而sPD-1水平两组比较无明显差异(P>0.05)。sPD-1、sPD-L1的表达水平与临床病理特征无明显相关(P>0.05),IFN-γ的表达水平与淋巴结转移情况相关(P<0.05),与T分期、TNM分期、肿瘤体积大小、肿瘤部位、组织分化程度、性别、年龄无明显相关(P>0.05)。血清中sPD-L1表达水平与IFN-γ无明显相关(P>0.05)。结论:食管鳞癌患者血清中sPD-L1较正常人表达升高,且术后表达较术前减少,说明血清中sPD-L1表达水平与病情发展变化有一定相关性。
Objective ： To evaluate the expression levels of programmed death- 1 （PD- 1）, programmed death ligand- 1 （PD-L1） and IFN-T in the peripheral blood of patients with esophageal squamous cell carcinoma （ESCC）, and to ex- plore their clinical implications. Methods ： A total of 90 ESCC patients （including 50 patients that underwent surgi- cal treatment） and 40 healthy controls were enrolled in the study. The periphery blood samples of the participants were collected and the expression levels of soluble PD-1 （sPD-1）, soluble PD-L 1 （sPD-L 1） and IFN-T in the serum were tested by enzyme-link immunology method. The data were tested and analyzed with SPSS 24.0 software. Re- sults： The expression levels of sPD-1,sPD-L 1 and IFN-γ in the blood of ESCC group were significantly higher than those in the healthy controls （P〈0.05）; the preoperative level of sPD-L1 and IFN-T in ESCC patients was significant- ly higher than that of postoperative level （P〈0.05）, whereas no significant difference was observed in the expression levels of sPD-1 before and after the surgery （P〉0.05）. sPD-1 and sPD-L1 expression had no remarkable correlation with clinicopathological manifestations （/9〉0.05）; however, IFN-γ expression was related to lymph node metastasis （P〈0.05）, but not with other manifestations （T stage, TNM stage, tumor size, tumor location, tumor cell differentia- tion, sex, and age） （P〉0.05）. The expression level of sPD-L1 had no significant correlation with IFN-γ （P〉0.05）. Conclusion： The serum level of sPD-L1 was higher in ESCC patients than that in healthy controls, and the post-op- erative sPD-L1 level was decreased compared with pre-operative level, indicating sPD-L1 may be associated with ESCC development.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2017年第10期1118-1123,共6页
Chinese Journal of Cancer Biotherapy
关键词
食管鳞状细胞癌
程序性死亡分子1
程序性死亡分子配体1
Γ-干扰素
esophageal squamous cell carcinoma
programmed cell death I（PD-1）
programmed cell death li-gand 1 （PD-L 1）
interferon-γ（IFN-γ）
