摘要
采用TEMPO/Na Br/Na Cl O体系对细菌纤维素(BC)进行氧化改性,然后以氧化细菌纤维素(OBC)、丝蛋白(SF)作为基础材料,负载骨形态发生蛋白(BMP-2),经电凝胶技术制备负载BMP-2的OBC/SF复合水凝胶。通过扫描电镜(SEM)、孔隙率、力学性能、体外降解率测试对所制备的复合水凝胶进行表征,将软骨细胞种植于负载BMP-2的OBC/SF复合水凝胶支架上进行体外联合培养,观察软骨细胞在支架中的增殖情况。结果表明:该OBC/SF复合水凝胶是一种具有细腻、疏松的双网络孔隙结构,良好的降解性能、优异的力学性能,适合细胞增殖的骨组织工程支架材料;BMP-2的加入有诱导软骨细胞分化,维持软骨细胞特点的作用。
The bacterial cellulose was modified by TEMPO/Na Br/Na Cl O-mediated oxidation. The oxidized bacterial cellulose/silk fibroin（ OBC/SF） composite hydrogel containing bone morphogenic protein（ BMP-2） for cartilage repair was prepared by gel electrophoresis. The prepared composite hydrogel was characterized by Scanning Electron Microscopy（ SEM）,porosity,mechanical testing and in-vitro degradation. The chondrocytes were grown on the prepared OBC/SF composite hydrogel scaffold for in-vitro culture. The growth of the chondrocytes on the composite scaffold was observed. Results show that the prepared OBC/SF composite hydrogel is an ideal scaffold for bone tissue engineering,with fine and loose double network structure,appropriate degradability,excellent mechanical properties and native environment for cell proliferation. Besides,the addition of BMP-2 contributes to the differentiation of chondrocytes and helps to maintain the characteristics of chondrocytes.
出处
《丝绸》
CAS
CSCD
北大核心
2017年第10期18-23,共6页
Journal of Silk
基金
江苏省高校自然科学基金项目(16KJB540005)
湖北省纺织新材料及其应用重点实验室开放课题(Fzxcl2017016)
生态纺织教育部重点实验室(江南大学)开放课题(KLET1601)
江苏省盐城市农业科技指导性计划项目(YKN2016012
YKN2016013)
江苏高校品牌专业建设工程资助项目(PPZY2015C254)
盐城工业职业技术学院院级重点课题(ygy201501)
江苏省高校优秀中青年教师境外研修计划项目(苏教师[2014]24号)
江苏省高等学校大学生创新创业训练计划项目(201713752017X)
