摘要
血管内皮细胞是位于循环血液与血管壁内壁之间的单层细胞,它在血栓与止血、炎症与免疫反应、血管新生和动脉粥样硬化等一系列生理和病理过程中发挥着重要作用。以合成高分子水凝胶为模型材料研究体外调控血管内皮细胞行为,对理解血管内皮细胞的生存微环境,进而优化设计适用于组织工程基础研究和应用需求的高分子水凝胶材料具有实质性意义。综述了调控血管内皮细胞行为的合成高分子水凝胶支架的研究工作,并对其未来发展进行了展望。介绍了无外来蛋白质修饰的条件下,可促进细胞扩增的活性合成高分子水凝胶细胞支架;以牛胎儿大动脉血管内皮细胞为例,讨论了高分子水凝胶的临界电位与调控细胞行为之间的关系;分析了调控细胞生存空间和动态运动行为的高分子水凝胶,并引入了集高强度、细胞亲和性于一体的高分子水凝胶的设计思路。
Endothelial cells are located on the inner surface of blood vessels as a monolayer,which separate the circulating blood from the tubular tissues.Endothelial cells play an important role in a series of physiological and pathological processes,such as thrombosis and hemostasis,inflammatory and immune response,angiogenesis as well as atherosclerosis.Manipulating cell behaviors in vitro using synthetic hydrogels as model materials is essential for understanding the microenvironment of endothelial cells and further designing hydrogels for basic research and potential application in tissue engineering.In this paper,we present a review on the advances in the synthetic hydrogel scaffolds that promote cell proliferation without surface modification of any cell adhesive proteins or peptides.The relationship between the critical Zeta potential of hydrogels and cell behaviors is discussed with bovine fetal aortic endothelial cells as an example.We also discuss the idea of designing hydrogels for manipulating living space and dynamic behaviors of endothelial cells,as well as that hydrogels possess both the biocompatibility and high strength for supporting proliferation of endothelial cells.Furthermore,the challenges,problems and future development of synthetic hydrogels as scaffolds to manipulate cell behaviors are proposed.
出处
《中国科技论文》
CAS
北大核心
2012年第9期666-673,共8页
China Sciencepaper
基金
国家自然科学基金资助项目(51073127,51173144)
高等学校博士学科点专项科研基金资助项目(20100201110040)
中央高校基本科研业务费资助项目(109-08140018)
留学回国人员科研启动基金
西安交通大学新教师科研支持计划资助项目(08141001)
高等学校学科创新引智计划项目(111计划B06024)
国家重大国际(地区)合作研究项目(11120101002)
