摘要
目的:采用高效液相色谱法(HPLC)建立安宫牛黄丸的指纹图谱,结合化学模式识别对其质量进行评价。方法:采用HPLC,以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱,检测波长230 nm,对10批安宫牛黄丸样品进行指纹图谱研究,通过相似度分析并结合主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)对安宫牛黄丸的质量进行评价。结果:10批样品相似度均>0.9,标定了25个色谱峰为共有峰,通过对照品比较指认了其中10个色谱峰。通过PCA发现不同年份样品存在微小差异,进一步采用OPLS-DA发现了影响不同年份样品产生差异的14种成分,包括盐酸巴马汀、黄芩素、表小檗碱、栀子苷、盐酸小檗碱、黄连碱、汉黄芩素等。结论:建立的分析方法简便、准确、可靠,指纹图谱结合化学模式识别分析可更好地评价安宫牛黄丸的质量,为该制剂的质量控制提供科学依据。
Objective： To establish an HPLC fingerprint of Angong Niuhuangwan and evaluate its quality by similarity calculation and chemical pattern recognition. Method： HPLC was uesd,the separation was performed with the mobile phase consisting of acetonitrile and 0. 1% phosphoric acid aqueous solution,and the detection wavelength was set at 230 nm. The fingerprints of 10 batches of Angong Niuhuangwan were analyzed by similarity analysis,and further evaluated the quality by principal component analysis（ PCA） and orthogonal partial least squares discriminant analysis（ OPLS-DA）. Result： HPLC fingerprint of Angong Niuhuangwan was established,the similarity was above 0. 9,and 25 peaks were recognized as common peaks,10 of them were identified by the reference substance. It was found that there were some differences among the samples from different years,and 14 differential components that causing differences in samples from different years were found by OPLSDA. Conclusion： The established analytical method is simple, accurate and reliable. The combination of fingerprint with chemical pattern recognition can evaluate the quality of Angong Niuhuangwan well and it can provide scientific basis for quality control of this preparation.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第20期14-19,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏省"333高层次人才培养工程"项目(BRA2016506)
关键词
安宫牛黄丸
指纹图谱
主成分分析
正交偏最小二乘法-判别分析
化学模式识别
盐酸小檗碱
相似度
Angong Niahaangwan
fingerprint
principal component analysis
orthogonal partial leastsquares discriminant analysis
chemical pattern recognition
berberine hydrochloride
similarity
