摘要
选用一株分枝杆菌(Mycobacterium sp.)限制性降解β-谷甾醇获得产物雄甾-4-烯-3,17-二酮(AD)和雄甾-1,4-二烯-3,17-二酮(ADD)。研究了培养基成分对产物产率的影响,在最佳pH 7.0,添加磷酸盐的条件下,得到了最高产率为89%。对β-谷甾醇的添加方式、添加量和补加时间的研究表明提高β-谷甾醇的有效反应浓度是提高转化效率的关键。
A strain of Mycobacterium sp. was used in degradation of β-sitosterol to androst-4-ene-3,17-dione (AD) and androst-1,4-diene-3,17-dione (ADD). Under the optimum conditions, the maximum production yield of AD(D) was up to 89%. In the course of degradation, pH and phosphate were important. The studies on the ways in which β-sitosterol was dispersed into the medium, the time when β-sitosterol was added and the amounts of β-sitosteron that were added suggested that the enhancement of the effective concentration of β-sitosterol be a key to the production of AD and ADD.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1991年第4期221-224,共4页
Journal of China Pharmaceutical University
关键词
β谷甾醇
微生物降解
侧链
Side chin degradation
β-Sitosterol
Androst-4-ene-3,17-dione (AD)
Androst-1,4-diene-3, 17-dione (ADD)
Mycobacterium sp.
