摘要
采用紫外线、亚硝基胍复合诱变雄甾-4-烯-3,17-二酮(AD)和雄甾-1,4-二烯-3,17-二酮(ADD)的转化产生菌Mycobacterium sp.,结合平板筛选,获得一株遗传性状稳定单产ADD的突变菌株Mycobacterium sp.-11,其ADD质量浓度达到1246 mg/L,比原始菌株(484 mg/L)提高了150%,经初步优化后发酵液中ADD最高达到1430 mg/L,发酵液中ADD质量占ADD、AD两产物质量总和的比例由70%提高到99.1%。
A mutant Mycobacterium sp. -11 was obtained by UV-NTG mutation and plate screening from a parent strain Mycobacterium sp. and it could transform sterol into androst-1,4-diene-3,17-dione （ADD） as the main product. The productivity of ADD was superior to that of the parental strain, the concentration of ADD increased from 484 mg/L to 1 246 mg/L. The highest concentration of ADD reached 1 430 mg/L by preliminary optimization. The conversion rate of ADD on sum of ADD and AD productivity was increased from 70% to 99. 1%.
出处
《生物加工过程》
CAS
CSCD
2009年第3期43-46,共4页
Chinese Journal of Bioprocess Engineering
基金
国家重点基础研究发展计划(973计划)资助项目(2007CB707804)
国家高技术研究发展计划(863计划)资助项目(2006AA020103)
国家自然科学基金资助项目(20676053)
教育部新世纪优秀人才支持计划资助项目(NCET-07-0380)
江苏省青年科技创新人才(学术带头人)基金资助项目(BK2006504)
