摘要
从海南罗非鱼的体内分离和鉴定出40株维氏气单胞菌,并对这40株维氏气单胞菌进行了8种毒力基因检测及ERIC-PCR分型分析.毒力基因检测的结果表明:在这40株维氏气单胞菌中,主要携带6种毒力基因,即act,alt,ast,fla,lip和hlyA基因,其携带率分别为35.0%,22.5%,27.5%,37.5%,5.0%和15%,未能检测到TapA和aerA基因;根据不同菌株携带毒力基因的不同,这40株维氏气单胞菌又可分为13种毒力基因型,携带2种以上毒的菌株有15株,占总数的37.5%,携带3种以上毒力基因的菌株有9株,占总数的22.5%,其中HNWC23携带5种毒力基因,其毒力基因型为act^+alt^+ast^+hly A^+fla^+.ERIC-PCR分型结果显示:该40株维氏气单胞菌可分为四个大簇,以遗传相似度(96%)为界可将其分为18种ERIC型,每个簇中的ERIC型都呈现多样性.
In the study,40 strains of Aeromonas veronii from Tilapia in Hainan Province were isolated and identi-fied,and eight virulence genes and ERIC-PCR sub-typing were analyzed.The results of virulence gene detection indicated that 6 virulence genes were detected and the carrying rate of 6 virulence genes of act,alt,ast,fla,lip and hlyA was 35.0%,22.5%,27.5%,37.5%,5.0%and 15%,respectively.However,the virulence genes TapA and aerA were not detected.The results of virulence gene genotyping demonstrated that there were 13 different virulence genotypes in 40 A.veronii strains,and 15 strains carried two or more virulence genotypes,accounting for 60%of total 40 strains,9 strains carried three or more virulence genotypes,accounting for 22.5%of total 40 strains.There were 5 virulence genes in HNWC23,whose virulence genotype was act+alt+ast+hlyA+fla+.The results of ERIC-PCR sub-typing suggested genotypes with 96%genetic similarity.that 40 strains can be divided into four clusters,18 genotypes with 96%genetic similarity.
作者
张永政
李聪
王世锋
蔡岩
周永灿
郑玉
朱薇薇
Zhang Yongzheng;Li Cong;Wang Shifeng;Cai Yan;Zhou Yongcan;Zheng Yu;Zhu Weiwei(State Key Laboratory of Marine Resource Utilization in South China Sea,Key Laboratory of Tropical Biological Resources of Ministry of Educa-tion,Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology,College of Marine Science,Hainan University,Haikou 570228,China)
出处
《海南大学学报(自然科学版)》
CAS
2017年第3期273-281,共9页
Natural Science Journal of Hainan University
基金
海南省科技兴海专项(2015XH02)
海洋药源生物种质资源库建设项目(12PYY001SF08-HNDX-1)
海南省重点科技计划项目(ZDXM20120005)
