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淡水养殖鱼类暴发性传染病致病细菌的研究 被引量:49

STUDIES ON THE TAXONOMY OF PATHOGENIC BACTERIA OF THE BACTERIAL HEMORRHAGIC SEPTICEMIA IN CULTURED FISHES IN FRESHWATER
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摘要 于1990—1991年的5—10月间,从湖北、湖南、河南和广东4省的9种淡水养殖鱼类的发病鱼睥、肝、肾中分离的93株细菌为材料,经100多个表型特征和细菌DNA中G+Cmol%遗传型特性测定等研究结果,可归属为产气单胞菌属中的运动性嗜中温产气单胞菌和弧菌属中的河弧菌。按照国际细菌命名法规优先律应为点状产气单胞菌[Aeromonas punctata(Zimmermann)nom.rev];而河弧菌与以前所描述的生物变种Ⅰ、Ⅱ型不同:在43℃能生长;发酵水杨苷产酸;利用D-葡糖酸钙、腐胺;不能利用戊二酸,D-葡糖醛酸;从D-葡萄糖产气;DNA中的G+Cmol%为47(Tm),鉴定为河弧菌生物变种Ⅲ(新生物变种Vibrio fluvialis Biovar Ⅲ.Biovar nov.)。 Taxonomic studies were performed on ninety-three bacterial isolates from nine species of fish affected by the septicemia and reared in fresh water in Hubei,Hunan, Henan and Guangdong during the period from May to October of 1990 and 1991. Analysing more than one hundred phenotypic characters and mol%G+C of the bacterial deoxyribonucleic acids, these isolates were assigned either to motile mesophilic aeromonad in the genus Aeromonas or to Vibrio fluvialis in the genus Vibrio. In conformity with the Priority of the International Code of Nomenclature of bacteria, these motile mesophilic aeromonads should be Aeromonas punctata(Zimmermann) nom. rev.; Another group of the pathogenic bacteria was similar to Vibrio fluvialis in most physiological and biochemical characteristics, but differs from the previously described V. fluvialis biovar Ⅰ and biovar Ⅱ in their grouth at 43℃, acid production from salicin, utilization of D-gluconate and putrescine, inability to utilizie glutarate and glucuronate, gas production from D-glucose and 47 mol% G+C of the base compositions. Therefore, a new biovar, Vibrio fluvialis biovar Ⅲ. biovar nov. is proposed.
出处 《水生生物学报》 CAS CSCD 北大核心 1993年第3期259-266,共8页 Acta Hydrobiologica Sinica
基金 国家自然科学基金 "八五"攻关资助项目
关键词 产气单胞菌 弧菌 淡水养殖 鱼纲 Bacterial hemorrhagic septicemia, Cultured fishes in freshwater, Aeromonas,Vibrio
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