摘要
目的探讨清肺益肾方联合左氧氟沙星片对肺炎克雷伯杆菌感染老龄大鼠免疫功能的影响及可能作用机制。方法 60只大鼠随机分为空白组、模型组、左氧氟沙星组及联合高、中、低剂量组,每组10只。除空白组外其余各组采用一次性气管插管滴菌法建立肺炎克雷伯杆菌感染老龄大鼠模型。造模成功第2天,空白组、模型组均给予生理盐水10 g/(kg·d)灌胃;联合高、中、低剂量组给予30.66、15.33、7.67 g/(kg·d)清肺益肾方提取液灌胃,同时每组均给予左氧氟沙星片混悬液42 mg/(kg·d)灌胃;左氧氟沙星组给予左氧氟沙星片混悬液42 mg/(kg·d)灌胃。各组给药6天后观察大鼠一般情况、肺组织病理变化、血清免疫球蛋白G(IgG)、免疫球蛋白A(IgA)表达和肺泡灌洗液中IgG、IgA、分泌型免疫型蛋白A(s IgA)变化。结果肺组织病理显示,模型组大鼠肺损伤较空白组明显,各治疗组肺损伤较模型组不同程度减轻。与空白组比较,模型组血清IgG、IgA和肺泡灌洗液IgA显著升高,肺泡灌洗液IgG、s IgA表达均显著下降(P<0.01);与模型组比较,左氧氟沙星组及联合高、中、低剂量组血清IgG、IgA表达和肺泡灌洗液IgA表达均显著下降,联合高、中剂量组肺泡灌洗液IgG、s IgA表达显著升高(P<0.05或P<0.01)。联合中剂量组血清IgG较联合高、低剂量组显著下降,且均显著低于左氧氟沙星组(P<0.01)。联合高、中剂量组肺泡灌洗液s IgA表达显著高于左氧氟沙星组(P<0.01)。结论清肺益肾方联合左氧氟沙星片可改善肺炎克雷伯菌杆菌感染老龄大鼠肺组织损伤,其机制可能与降低外周血炎症反应及升高肺脏局部免疫力有关。
Objective To explore the impact and possible mechanism of Qingfei Yishen Fang （清肺益肾方）combined with levofloxacin tablet on immune function of aged rats’ infected by Klebsiella pneumonia.Methods Sixty rats were randomized into the blank group,the model group,the levofloxacin group,the combination of high,median and low dose group,10 rats in each group.Rats in other groups were made models of aged rats infected by Klebsiella pneumonia by disposable tracheal intubation method of dropping bacteria except the blank group.On the 2 nd day of successful modeling,the blank group and the model group were given normal saline 10 g/ （kg · d） by gavage.The combination of high,median and low dose groups were given Qingfei Yishen Fang extract 30.66,15.33,7.67 g/ （kg·d） by gavage respectively; meanwhile,each group was given levofloxacin tablet suspension 42mg/ （kg·d） by gavage.The levofloxacin group was given evofloxacin tablet suspension 42 mg/ （kg·d） by gavage.After administration for 6 days,it was examined in each group that rats’ general condition,pathological changes of lung tissue,the expressions of serum immunoglobulin G （IgG） and immunoglobulin A （IgA）,as well as the changes of IgG,IgA and secretory immunoglobulin A （s IgA） in broncho-alveolar lavage fluid.Results Lung tissue pathology showed that rat lung injury in the model group was heavier than that in the blank group.Rat lung injury in the treating groups got varying degrees of relief compared with the model group.Compared with the blank group,serum IgG,IgA and broncho-alveolar lavage fluid IgA in the model group increased; while the expressions of broncho-alveolar lavage fluid IgG and s IgA decreased （P 〈 0.01）.Compared with the model group,the expressions of serum IgG,IgA and broncho-alveolar lavage fluid IgA in the levofloxacin group,the combination of high,median and low dose groups decreased.The expressions of broncho-alveolar lavage fluid IgG and s IgA increased in the combination of high and median dose groups （P 〈 0.05 or P 〈 0.01）.Serum IgG in the combination of median dose group was lower than that in the combination of high and low dose groups.Serum IgG in the combination groups was lower than that in the levofloxacin group （P 〈 0.01）.The expression of broncho-alveolar lavage fluid s IgA in the combination of high and median dose groups was higher than that in the levofloxacin group （P 〈 0.01）.Conclusion Qingfei Yishen Fang combined with levofloxacin tablet might improve the aged rats’ lung tissue injury infected by Klebsiella pneumonia.The mechanism might relate to reducing peripheral blood inflammatory response and improving the local immunity of lung.
作者
孟泳
崔应麟
芦晓帆
MENG Yong CUI Yinglin LU Xiaofan(He＇nan University of Chinese Medicine, Zhengzhou, 45000)
出处
《中医杂志》
CSCD
北大核心
2017年第19期1681-1685,共5页
Journal of Traditional Chinese Medicine
基金
河南省重点科技攻关计划(152102310107)
关键词
细菌性肺炎
老年
清肺益肾方
左氧氟沙星片
免疫功能
克雷伯杆菌感染
bacterial pneumonia
the aged
Qingfei Yishen Fang
levofloxacin tablet
immunologic function
Klebsiella pneumonia infection
