摘要
以太行菊为试材,以超声波辅助提取试验,采用单因素分析和响应面分析相结合的方法,筛选出最优的太行菊黄酮提取组合配置,针对太行菊黄酮类化合物提取工艺进行优化。结果表明:单因素试验中最佳的料液比1∶25g·mL^(-1),乙醇浓度60%,提取温度60℃,提取时间45min。响应面试验的方差分析表明,料液比、乙醇浓度和提取温度对黄酮提取率影响差异极显著,提取时间对提取率的影响差异不显著。4个因素对提取率的影响依次为料液比>乙醇浓度>提取温度>提取时间。回归方程模型预测的最优提取工艺条件为料液比1∶25.88g·mL^(-1)、乙醇浓度61.78%、提取温度63.44℃、提取时间55min,太行菊黄酮提取率可达极值4.74%。料液比、乙醇浓度和提取温度的增加极显著的提高了太行菊黄酮的提取率。
Taking Opisthopapus taihangensis as test material,the single-factor test was carried out by ultrasonic-assisted extraction.The optimum extraction process of flavonoids from Opisthopapus taihangensis was analyzed by Box-Behnken response surface.The results showed that the extraction conditions were as follows the ratio of material to liquid 1∶25g·mL^（-1）,ethanol concentration 60%,extraction temperature 60℃,extraction time 45 minutes.The variance analysis of the response surface test showed that solid-liquid ratio,ethanol concentration and extraction temperature on the extraction rate of flavonoids was very significant,and the extraction time had no significant effect on the extraction rate of flavonoids.The effects of four factors on the extraction rate were as follows：solid-liquid ratioethanol concentrationextraction temperatureextraction time.The optimal extraction conditions were as follows,solid-liquid ratio was 1∶25.88g· mL^（-1）,the concentration of ethanol was 61.78%,the extraction temperature was 63.44 ℃,the extraction time was 55 minutes,and the extraction rate of flavonoids was 4.74%.The solid-liquid ratio,ethanol concentration and extraction temperature could increase the extraction rate of flavonoids fromOpisthopapus taihangensis very significantly.
出处
《北方园艺》
CAS
北大核心
2017年第18期131-137,共7页
Northern Horticulture
基金
河北省科技计划资助项目(13222907)
邯郸市科技局资助项目(1627201054)
邯郸学院校级资助项目(15202
16211)
关键词
太行菊
黄酮
超声辅助提取
单因素试验
响应面法
Opisthopapus taihangensis
flavonoids
ultrasonic-assisted extraction extraction
single-factor experiment
response surface method
